Unique and differential protein signatures within the mononuclear cells of HIV-1 and hcv mono-infected and co-infected patients

Nawal M. Boukli, Vivekananda Shetty, Luis Cubano, Martha Ricaurte, Jordana Coelho-Dos-Reis, Zacharie Nickens, Punit Shah, Andrew H. Talal, Ramila Philip, Pooja Jain

Research output: Contribution to journalArticle

Abstract

Background: Pathogenesis of liver damage in patients with HIV and HCV co-infection is complex and multifactorial. Although global awareness regarding HIV-1/HCV co-infection is increasing little is known about the pathophysiology that mediates the rapid progression to hepatic disease in the co-infected individuals. Results: In this study, we investigated the proteome profiles of peripheral blood mononuclear cells from HIV-1 mono-, HCV mono-, and HIV-1/HCV co-infected patients. The results of high-resolution 2D gel electrophoresis and PD quest software quantitative analysis revealed that several proteins were differentially expressed in HIV-1, HCV, and HIV-1/HCV coinfection. Liquid chromatography-mass spectrometry and Mascot database matching (LC-MS/MS analysis) successfully identified 29 unique and differentially expressed proteins. These included cytoskeletal proteins (tropomyosin, gelsolin, DYPLSL3, DYPLSL4 and profilin-1), chaperones and co-chaperones (HSP90-beta and stress-induced phosphoprotein), metabolic and pre-apoptotic proteins (guanosine triphosphate [GTP]-binding nuclear protein Ran, the detoxifying enzyme glutathione S-transferase (GST) and Rho GDP-dissociation inhibitor (Rho-GDI), proteins involved in cell prosurvival mechanism, and those involved in matrix synthesis (collagen binding protein 2 [CBP2]). The six most significant and relevant proteins were further validated in a group of mono- And co-infected patients (n = 20) at the transcriptional levels. Conclusions: The specific pro- And anti- Apoptotic protein signatures revealed in this study could facilitate the understanding of apoptotic and protective immune-mediated mechanisms underlying HIV-1 and HCV co-infection and their implications on liver disease progression in co-infected patients.

Original languageEnglish (US)
Article number11
JournalClinical Proteomics
Volume9
Issue number1
DOIs
StatePublished - 2012

Keywords

  • 2D-GE
  • HCV
  • HIV-1
  • HIV-1/HCV
  • Mass spectrometry
  • Pro-and anti-apoptotic fingerprinting
  • Proteomics

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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    Boukli, N. M., Shetty, V., Cubano, L., Ricaurte, M., Coelho-Dos-Reis, J., Nickens, Z., Shah, P., Talal, A. H., Philip, R., & Jain, P. (2012). Unique and differential protein signatures within the mononuclear cells of HIV-1 and hcv mono-infected and co-infected patients. Clinical Proteomics, 9(1), [11]. https://doi.org/10.1186/1559-0275-9-11