Uninduced high-yield bacterial expression of fluorescent proteins

Sarvenaz Sarabipour, Christopher King, Kalina A Hristova

Research output: Contribution to journalArticle

Abstract

Here we introduce a fast, cost-effective, and highly efficient method for production of soluble fluorescent proteins from bacteria. The method does not require optimization and does not use isopropyl β-d-1-thiogalactopyranoside (IPTG) induction. The method relies on uninduced expression in the BL21-Gold (DE3) strain of Escherichia coli and yields large amounts (up to 0.4 μmol) of fluorescent protein from a 250-ml culture. This method is much simpler than published methods and can be used to produce any fluorescent protein that is needed in biomedical research.

Original languageEnglish (US)
Pages (from-to)155-157
Number of pages3
JournalAnalytical Biochemistry
Volume449
Issue number1
DOIs
StatePublished - Mar 15 2014

Fingerprint

Thiogalactosides
Proteins
Gold
Escherichia coli
Bacteria
Biomedical Research
Costs
Costs and Cost Analysis

Keywords

  • E. coli
  • Fluorescent protein
  • FRET
  • Gene expression
  • High yield
  • His-tag
  • Protein production

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Uninduced high-yield bacterial expression of fluorescent proteins. / Sarabipour, Sarvenaz; King, Christopher; Hristova, Kalina A.

In: Analytical Biochemistry, Vol. 449, No. 1, 15.03.2014, p. 155-157.

Research output: Contribution to journalArticle

Sarabipour, Sarvenaz ; King, Christopher ; Hristova, Kalina A. / Uninduced high-yield bacterial expression of fluorescent proteins. In: Analytical Biochemistry. 2014 ; Vol. 449, No. 1. pp. 155-157.
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