Uninduced high-yield bacterial expression of fluorescent proteins

Sarvenaz Sarabipour, Christopher King, Kalina Hristova

Research output: Contribution to journalArticlepeer-review

Abstract

Here we introduce a fast, cost-effective, and highly efficient method for production of soluble fluorescent proteins from bacteria. The method does not require optimization and does not use isopropyl β-d-1-thiogalactopyranoside (IPTG) induction. The method relies on uninduced expression in the BL21-Gold (DE3) strain of Escherichia coli and yields large amounts (up to 0.4 μmol) of fluorescent protein from a 250-ml culture. This method is much simpler than published methods and can be used to produce any fluorescent protein that is needed in biomedical research.

Original languageEnglish (US)
Pages (from-to)155-157
Number of pages3
JournalAnalytical biochemistry
Volume449
Issue number1
DOIs
StatePublished - Mar 15 2014

Keywords

  • E. coli
  • FRET
  • Fluorescent protein
  • Gene expression
  • High yield
  • His-tag
  • Protein production

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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