Unequal synthesis of globin chains after extended incubation of rabbit reticulocytes with l-O-methylthreonine

Monte A. Del Monte, Haig H. Kazazian

Research output: Contribution to journalArticlepeer-review

Abstract

Rabbit reticulocytes were incubated in vitro as long as 20 hr with l-O-methyl threonine, the OMT was removed, and the capacity of the cells to synthesize α- and β-globin chains was determined. After 20 hr incubation in vitro, OMT-treated cells can make β chains about twice as well as cells incubated for 20 hr without OMT. However, the capacity of OMT-treated cells to synthesize α chains was only about 60% that of cells incubated without OMT for 20 hr. These results suggest that the capacity of reticulocytes to produce a specific globin chain after an in vitro incubation correlates positively with the number of ribosomes associated with the specific messenger RNA for that chain during the incubation.

Original languageEnglish (US)
Pages (from-to)429-434
Number of pages6
JournalJournal of molecular biology
Volume56
Issue number2
DOIs
StatePublished - Mar 14 1971

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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