TY - JOUR
T1 - Understanding the Molecular Mechanisms of the CRISPR Toolbox Using Single Molecule Approaches
AU - Singh, Digvijay
AU - Ha, Taekjip
N1 - Funding Information:
The project was supported by grants from National Science Foundation (PHY-1430124 to T.H.) and National Institutes of Health (GM065367; GM112659 to T.H). T.H. is an investigator with the Howard Hughes Medical Institute.
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/3/16
Y1 - 2018/3/16
N2 - Adaptive immunity against foreign genetic elements conferred by the CRISPR systems in microbial species has been repurposed as a revolutionary technology for wide-ranging biological applications - chiefly genome engineering. Biochemical, structural, genetic, and genomics studies have revealed important insights into their function and mechanisms, but most ensemble studies cannot observe structural changes of these molecules during their function and are often blind to key reaction intermediates. Here, we review the use of single molecule approaches such as fluorescent particle tracking, FRET, magnetic tweezers, and atomic force microscopy imaging in improving our understanding of the CRISPR toolbox.
AB - Adaptive immunity against foreign genetic elements conferred by the CRISPR systems in microbial species has been repurposed as a revolutionary technology for wide-ranging biological applications - chiefly genome engineering. Biochemical, structural, genetic, and genomics studies have revealed important insights into their function and mechanisms, but most ensemble studies cannot observe structural changes of these molecules during their function and are often blind to key reaction intermediates. Here, we review the use of single molecule approaches such as fluorescent particle tracking, FRET, magnetic tweezers, and atomic force microscopy imaging in improving our understanding of the CRISPR toolbox.
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U2 - 10.1021/acschembio.7b00905
DO - 10.1021/acschembio.7b00905
M3 - Review article
C2 - 29394047
AN - SCOPUS:85044062149
SN - 1554-8929
VL - 13
SP - 516
EP - 526
JO - ACS chemical biology
JF - ACS chemical biology
IS - 3
ER -