Understanding the Molecular Mechanisms of the CRISPR Toolbox Using Single Molecule Approaches

Digvijay Singh, Taekjip Ha

Research output: Contribution to journalReview article

Abstract

Adaptive immunity against foreign genetic elements conferred by the CRISPR systems in microbial species has been repurposed as a revolutionary technology for wide-ranging biological applications - chiefly genome engineering. Biochemical, structural, genetic, and genomics studies have revealed important insights into their function and mechanisms, but most ensemble studies cannot observe structural changes of these molecules during their function and are often blind to key reaction intermediates. Here, we review the use of single molecule approaches such as fluorescent particle tracking, FRET, magnetic tweezers, and atomic force microscopy imaging in improving our understanding of the CRISPR toolbox.

Original languageEnglish (US)
Pages (from-to)516-526
Number of pages11
JournalACS Chemical Biology
Volume13
Issue number3
DOIs
StatePublished - Mar 16 2018

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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