Abstract
Protein kinase phosphorylation is central to the regulation and control of protein and cellular function. Over the past decade, the development of many high-throughput approaches has revolutionized the understanding of protein phosphorylation and allowed rapid and unbiased surveys of phosphoproteins and phosphorylation events. In addition to this technological advancement, there have also been computational improvements; recent studies on network models of protein phosphorylation have provided many insights into the cellular processes and pathways regulated by phosphorylation. This article gives an overview of experimental and computational techniques for identifying and analyzing protein phosphorylation on a systems level.
Original language | English (US) |
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Article number | elp045 |
Pages (from-to) | 32-42 |
Number of pages | 11 |
Journal | Briefings in Functional Genomics and Proteomics |
Volume | 9 |
Issue number | 1 |
DOIs | |
State | Published - Jan 7 2010 |
Keywords
- Kinase
- Mass spectrometry
- Phosphoprotein
- Protein microarray
- Protein phosphorylation
- Systems biology
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics