Understanding nanotopography induced stem cell differentiation: A focus on focal adhesion Kinase

Benjamin Kim Kiat Teo, Sum Thai Wong, Choon Kiat Lim, Terrence Y.S. Kung, Chong Hao Yap, Yamini Ramgopal, Lewis H. Romer, Evelyn K.F. Yim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biophysical cues, such as nanotopography, have been shown to be integral for tissue regeneration and embryogenesis in the stem cell niche. Synthetic nanostructures can drive specific cell differentiation, but the sensing mechanisms for nanocues remain poorly understood. Here, we report that nanotopography-induced human mesenchymal stem cell (hMSC) differentiation through cell mechanotransduction is modulated by the integrin-activated focal adhesion kinase (FAK). On 250 nm nanogratings on polydimethylsiloxane, hMSCs developed aligned stress fibers and showed an upregulation of neurogenic differentiation markers. In addition, our mechanistic study confirmed that this regulation was dependent upon actomyosin contractility, suggesting a direct forcedependent mechanism. FAK phosphorylation was required for topography-induced hMSC differentiation while FAK overexpression overruled the topographical cues in determining cell lineage bias. The results indicated that FAK activity had a direct impact on topography-induced gene expression. These findings suggest that hMSC sense and transduce nanotopographical signals through focal adhesions and actomyosin cytoskeleton contractility to induce differential gene expression.

Original languageEnglish (US)
Title of host publicationThe 15th International Conference on Biomedical Engineering, ICBME 2013
EditorsJames Goh
PublisherSpringer Verlag
Pages36-39
Number of pages4
ISBN (Electronic)9783319029122
DOIs
StatePublished - Jan 1 2014
Event15th International Conference on Biomedical Engineering, ICBME 2013 - Singapore, Singapore
Duration: Dec 4 2013Dec 7 2013

Publication series

NameIFMBE Proceedings
Volume43
ISSN (Print)1680-0737

Other

Other15th International Conference on Biomedical Engineering, ICBME 2013
CountrySingapore
CitySingapore
Period12/4/1312/7/13

Keywords

  • Cell signaling
  • Differentiation
  • FAK
  • Focal adhesions
  • Mesenchymal stem cell
  • Physical cues

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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  • Cite this

    Teo, B. K. K., Wong, S. T., Lim, C. K., Kung, T. Y. S., Yap, C. H., Ramgopal, Y., Romer, L. H., & Yim, E. K. F. (2014). Understanding nanotopography induced stem cell differentiation: A focus on focal adhesion Kinase. In J. Goh (Ed.), The 15th International Conference on Biomedical Engineering, ICBME 2013 (pp. 36-39). (IFMBE Proceedings; Vol. 43). Springer Verlag. https://doi.org/10.1007/978-3-319-02913-9_10