TY - JOUR
T1 - Uncoupling translocation from translation
T2 - Implications for transport of proteins across membranes
AU - Perara, Eve
AU - Rothman, Richard E.
AU - Lingappa, Vishwanath R.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1986
Y1 - 1986
N2 - The segregation of secretory proteins into the cisternae of the endoplasmic reticulum (ER) is normally tightly coupled to their synthesis. This feature distinguishes their biogenesis from that of proteins targeted to many other organelles. In the examples presented, translocation across the ER membrane is dissociated from translation. Transport, which is normally cotranslational, may proceed in the absence of chain elongation. Moreover, translocation across the ER membrane does not proceed spontaneously since, even in the absence of protein synthesis, energy substrates are required for translocation. These conclusions have been extended to the cotranslational integration of newly synthesized transmembrane proteins.
AB - The segregation of secretory proteins into the cisternae of the endoplasmic reticulum (ER) is normally tightly coupled to their synthesis. This feature distinguishes their biogenesis from that of proteins targeted to many other organelles. In the examples presented, translocation across the ER membrane is dissociated from translation. Transport, which is normally cotranslational, may proceed in the absence of chain elongation. Moreover, translocation across the ER membrane does not proceed spontaneously since, even in the absence of protein synthesis, energy substrates are required for translocation. These conclusions have been extended to the cotranslational integration of newly synthesized transmembrane proteins.
UR - http://www.scopus.com/inward/record.url?scp=0022550043&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022550043&partnerID=8YFLogxK
U2 - 10.1126/science.3961485
DO - 10.1126/science.3961485
M3 - Article
C2 - 3961485
AN - SCOPUS:0022550043
SN - 0036-8075
VL - 232
SP - 348
EP - 352
JO - Science
JF - Science
IS - 4748
ER -