Uncoupling apical constriction from tissue invagination

Se Yeon Chung, Sangjoon Kim, Deborah J. Andrew

Research output: Contribution to journalArticlepeer-review

Abstract

Apical constriction is a widely utilized cell shape change linked to folding, bending and invagination of polarized epithelia. It remains unclear how apical constriction is regulated spatiotemporally during tissue invagination and how this cellular process contributes to tube formation in different developmental contexts. Using Drosophila salivary gland (SG) invagination as a model, we show that regulation of folded gastrulation expression by the Fork head transcription factor is required for apicomedial accumulation of Rho kinase and non-muscle myosin II, which coordinate apical constriction. We demonstrate that neither loss of spatially coordinated apical constriction nor its complete blockage prevent internalization and tube formation, although such manipulations affect the geometry of invagination. When apical constriction is disrupted, compressing force generated by a tissue-level myosin cable contributes to SG invagination. We demonstrate that fully elongated polarized SGs can form outside the embryo, suggesting that tube formation and elongation are intrinsic properties of the SG.

Original languageEnglish (US)
JournaleLife
Volume6
DOIs
StatePublished - Mar 6 2017

Keywords

  • apical constriction
  • cell biology
  • D. melanogaster
  • developmental biology
  • Drosophila
  • fog
  • fork head
  • myosin
  • salivary gland
  • stem cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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