Uncommon TERT promoter mutations in pediatric thyroid cancer

Ali S. Alzahrani, Ebtesam Qasem, Avaniyapuram Kannan Murugan, Hindi N. Al-Hindi, Dania Alkhafaji, Mai Almohanna, Michael Mingzhao Xing, Doha Alhomaidah, Meshael Alswailem

Research output: Contribution to journalArticle

Abstract

Purpose: The aim of this study was to determine the rate and significance of TERT promoter mutations that have been recently described in adult thyroid cancer (TC) but not yet in the uncommonly occurring pediatric TC. Furthermore, the role of the BRAFV600E mutation in the clinical outcome of pediatric TC is unknown. Method: The study included 55 pediatric (median age 16 years, range 9-18 years; 46 females) and 210 adult TC patients (median age 40 years, range 20-75 years; 155 females) seen during the same time period. DNA was isolated from TC tissues and subjected to direct sequencing. Genetic-clinicopathological correlations were analyzed. Results: Only one case of pediatric TC was found to harbor the C228T TERT promoter mutation (1.8%). The C250T mutation was not detected in any of the 55 pediatric TC. In contrast, there was a significantly higher rate of TERT promoter mutations in the adult patients (15.7%, 33/210) compared with the pediatric patients (p = 0.003). In addition, persistent/recurrent TC was seen in 8/12 (66.7%) pediatric patients harboring the BRAFV600E mutation versus 14/41 (34.1%) patients harboring the wild type BRAF (p = 0.05), and when only conventional papillary TC was examined, in 7/9 (77.8%) cases harboring BRAFV600E mutation versus 11/33 (33.3%) cases harboring wild type BRAF (p = 0.025). Conclusions: This is the first study on TERT promoter mutations in pediatric TC, which revealed an exceedingly low prevalence, suggesting a limited role of these mutations in pediatric TC. This study also for the first time demonstrates an association of the BRAFV600E mutation with TC recurrence in pediatric patients.

Original languageEnglish (US)
Pages (from-to)235-241
Number of pages7
JournalThyroid
Volume26
Issue number2
DOIs
StatePublished - Feb 1 2016

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Thyroid Neoplasms
Pediatrics
Mutation
Recurrence
DNA

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Alzahrani, A. S., Qasem, E., Murugan, A. K., Al-Hindi, H. N., Alkhafaji, D., Almohanna, M., ... Alswailem, M. (2016). Uncommon TERT promoter mutations in pediatric thyroid cancer. Thyroid, 26(2), 235-241. https://doi.org/10.1089/thy.2015.0510

Uncommon TERT promoter mutations in pediatric thyroid cancer. / Alzahrani, Ali S.; Qasem, Ebtesam; Murugan, Avaniyapuram Kannan; Al-Hindi, Hindi N.; Alkhafaji, Dania; Almohanna, Mai; Xing, Michael Mingzhao; Alhomaidah, Doha; Alswailem, Meshael.

In: Thyroid, Vol. 26, No. 2, 01.02.2016, p. 235-241.

Research output: Contribution to journalArticle

Alzahrani, AS, Qasem, E, Murugan, AK, Al-Hindi, HN, Alkhafaji, D, Almohanna, M, Xing, MM, Alhomaidah, D & Alswailem, M 2016, 'Uncommon TERT promoter mutations in pediatric thyroid cancer', Thyroid, vol. 26, no. 2, pp. 235-241. https://doi.org/10.1089/thy.2015.0510
Alzahrani AS, Qasem E, Murugan AK, Al-Hindi HN, Alkhafaji D, Almohanna M et al. Uncommon TERT promoter mutations in pediatric thyroid cancer. Thyroid. 2016 Feb 1;26(2):235-241. https://doi.org/10.1089/thy.2015.0510
Alzahrani, Ali S. ; Qasem, Ebtesam ; Murugan, Avaniyapuram Kannan ; Al-Hindi, Hindi N. ; Alkhafaji, Dania ; Almohanna, Mai ; Xing, Michael Mingzhao ; Alhomaidah, Doha ; Alswailem, Meshael. / Uncommon TERT promoter mutations in pediatric thyroid cancer. In: Thyroid. 2016 ; Vol. 26, No. 2. pp. 235-241.
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abstract = "Purpose: The aim of this study was to determine the rate and significance of TERT promoter mutations that have been recently described in adult thyroid cancer (TC) but not yet in the uncommonly occurring pediatric TC. Furthermore, the role of the BRAFV600E mutation in the clinical outcome of pediatric TC is unknown. Method: The study included 55 pediatric (median age 16 years, range 9-18 years; 46 females) and 210 adult TC patients (median age 40 years, range 20-75 years; 155 females) seen during the same time period. DNA was isolated from TC tissues and subjected to direct sequencing. Genetic-clinicopathological correlations were analyzed. Results: Only one case of pediatric TC was found to harbor the C228T TERT promoter mutation (1.8{\%}). The C250T mutation was not detected in any of the 55 pediatric TC. In contrast, there was a significantly higher rate of TERT promoter mutations in the adult patients (15.7{\%}, 33/210) compared with the pediatric patients (p = 0.003). In addition, persistent/recurrent TC was seen in 8/12 (66.7{\%}) pediatric patients harboring the BRAFV600E mutation versus 14/41 (34.1{\%}) patients harboring the wild type BRAF (p = 0.05), and when only conventional papillary TC was examined, in 7/9 (77.8{\%}) cases harboring BRAFV600E mutation versus 11/33 (33.3{\%}) cases harboring wild type BRAF (p = 0.025). Conclusions: This is the first study on TERT promoter mutations in pediatric TC, which revealed an exceedingly low prevalence, suggesting a limited role of these mutations in pediatric TC. This study also for the first time demonstrates an association of the BRAFV600E mutation with TC recurrence in pediatric patients.",
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AU - Alzahrani, Ali S.

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AU - Al-Hindi, Hindi N.

AU - Alkhafaji, Dania

AU - Almohanna, Mai

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AU - Alswailem, Meshael

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N2 - Purpose: The aim of this study was to determine the rate and significance of TERT promoter mutations that have been recently described in adult thyroid cancer (TC) but not yet in the uncommonly occurring pediatric TC. Furthermore, the role of the BRAFV600E mutation in the clinical outcome of pediatric TC is unknown. Method: The study included 55 pediatric (median age 16 years, range 9-18 years; 46 females) and 210 adult TC patients (median age 40 years, range 20-75 years; 155 females) seen during the same time period. DNA was isolated from TC tissues and subjected to direct sequencing. Genetic-clinicopathological correlations were analyzed. Results: Only one case of pediatric TC was found to harbor the C228T TERT promoter mutation (1.8%). The C250T mutation was not detected in any of the 55 pediatric TC. In contrast, there was a significantly higher rate of TERT promoter mutations in the adult patients (15.7%, 33/210) compared with the pediatric patients (p = 0.003). In addition, persistent/recurrent TC was seen in 8/12 (66.7%) pediatric patients harboring the BRAFV600E mutation versus 14/41 (34.1%) patients harboring the wild type BRAF (p = 0.05), and when only conventional papillary TC was examined, in 7/9 (77.8%) cases harboring BRAFV600E mutation versus 11/33 (33.3%) cases harboring wild type BRAF (p = 0.025). Conclusions: This is the first study on TERT promoter mutations in pediatric TC, which revealed an exceedingly low prevalence, suggesting a limited role of these mutations in pediatric TC. This study also for the first time demonstrates an association of the BRAFV600E mutation with TC recurrence in pediatric patients.

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