UNC-80 and the NCA Ion Channels Contribute to Endocytosis Defects in Synaptojanin Mutants

Maelle Jospin, Shigeki Watanabe, Deepa Joshi, Sean Young, Kevin Hamming, Colin Thacker, Terrance P. Snutch, Erik M. Jorgensen, Kim Schuske

Research output: Contribution to journalArticle

Abstract

Synaptojanin is a lipid phosphatase required to degrade phosphatidylinositol 4,5 bisphosphate (PIP2) at cell membranes during synaptic vesicle recycling [1, 2]. Synaptojanin mutants in C. elegans are severely uncoordinated and are depleted of synaptic vesicles, possibly because of accumulation of PIP2 [2]. To identify proteins that act downstream of PIP2 during endocytosis, we screened for suppressors of synaptojanin mutants in the nematode C. elegans. A class of uncoordinated mutants called "fainters" partially suppress the locomotory, vesicle depletion, and electrophysiological defects in synaptojanin mutants. These suppressor loci include the genes for the NCA ion channels [3], which are homologs of the vertebrate cation leak channel NALCN [4], and a novel gene called unc-80. We demonstrate that unc-80 encodes a novel, but highly conserved, neuronal protein required for the proper localization of the NCA-1 and NCA-2 ion channel subunits. These data suggest that activation of the NCA ion channel in synaptojanin mutants leads to defects in recycling of synaptic vesicles.

Original languageEnglish (US)
Pages (from-to)1595-1600
Number of pages6
JournalCurrent Biology
Volume17
Issue number18
DOIs
StatePublished - Sep 18 2007
Externally publishedYes

Fingerprint

ion channels
endocytosis
Endocytosis
Ion Channels
Synaptic Vesicles
mutants
Defects
recycling
Recycling
Genes
phosphatidylinositols
Cell membranes
Phosphatidylinositols
Phosphoric Monoester Hydrolases
cell membranes
Vertebrates
Cations
cations
Proteins
genes

Keywords

  • CELLBIO
  • MOLNEURO

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Jospin, M., Watanabe, S., Joshi, D., Young, S., Hamming, K., Thacker, C., ... Schuske, K. (2007). UNC-80 and the NCA Ion Channels Contribute to Endocytosis Defects in Synaptojanin Mutants. Current Biology, 17(18), 1595-1600. https://doi.org/10.1016/j.cub.2007.08.036

UNC-80 and the NCA Ion Channels Contribute to Endocytosis Defects in Synaptojanin Mutants. / Jospin, Maelle; Watanabe, Shigeki; Joshi, Deepa; Young, Sean; Hamming, Kevin; Thacker, Colin; Snutch, Terrance P.; Jorgensen, Erik M.; Schuske, Kim.

In: Current Biology, Vol. 17, No. 18, 18.09.2007, p. 1595-1600.

Research output: Contribution to journalArticle

Jospin, M, Watanabe, S, Joshi, D, Young, S, Hamming, K, Thacker, C, Snutch, TP, Jorgensen, EM & Schuske, K 2007, 'UNC-80 and the NCA Ion Channels Contribute to Endocytosis Defects in Synaptojanin Mutants', Current Biology, vol. 17, no. 18, pp. 1595-1600. https://doi.org/10.1016/j.cub.2007.08.036
Jospin, Maelle ; Watanabe, Shigeki ; Joshi, Deepa ; Young, Sean ; Hamming, Kevin ; Thacker, Colin ; Snutch, Terrance P. ; Jorgensen, Erik M. ; Schuske, Kim. / UNC-80 and the NCA Ion Channels Contribute to Endocytosis Defects in Synaptojanin Mutants. In: Current Biology. 2007 ; Vol. 17, No. 18. pp. 1595-1600.
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