Ultraviolet irradiation produces novel endonuclease III-sensitive cytosine photoproducts at dipyrimidine sites

J. Jen, D. L. Mitchell, R. P. Cunningham, C. A. Smith, J. S. Taylor, J. E. Cleaver

Research output: Contribution to journalArticle

Abstract

Ultraviolet light irradiation of DNA in vitro and in vivo induces cyclobutane dimers, (6-4) pyrimidine-pyrimidone photoproducts and a variety of minor products. Using a defined DNA fragment, we have identified two classes of sites that can be cleaved by Escherichia coli endonuclease III: single cytosines whose heat lability corresponds to that of cytosine hydrates and more heat-stable dipyrimidines containing cytosine. The dipyrimidine products are induced at sites suggestive of (6-4) photoproducts but are not recognized as (6-4) photoproducts by radioimmunoassay. Use of oligonucleotides containing a single cyclobutane thymine dimer, a (6-4) photoproduct or the Dewar photoisomer of the (6-4) photoproduct also indicated that these products are not substrates for endonuclease III. We have therefore identified a minor UV photoproduct that has the same sequence specificity as the two major dipyrimidine photoproducts; it may be a minor isomer, a unique derivative or an oxidative lesion confined to dipyrimidine sites. Its biological significance is not yet known but may be masked by the preponderance of major products at the same sites. Its occurrence at the particular site in dipyrimidine sequences involved in the mutagenic action of UV photoproducts suggests that it may play a role in generating C to T transitions that are common UV-induced mutations.

Original languageEnglish (US)
Pages (from-to)323-329
Number of pages7
JournalPhotochemistry and Photobiology
Volume65
Issue number2
StatePublished - Feb 1997

Fingerprint

Endonucleases
Cytosine
Irradiation
cyclobutane
irradiation
products
Hot Temperature
Cyclobutanes
Dewars
Pyrimidine Dimers
deoxyribonucleic acid
DNA
dimers
radioimmunoassay
Ultraviolet Rays
Hydrates
Oligonucleotides
Isomers
heat
Dimers

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Jen, J., Mitchell, D. L., Cunningham, R. P., Smith, C. A., Taylor, J. S., & Cleaver, J. E. (1997). Ultraviolet irradiation produces novel endonuclease III-sensitive cytosine photoproducts at dipyrimidine sites. Photochemistry and Photobiology, 65(2), 323-329.

Ultraviolet irradiation produces novel endonuclease III-sensitive cytosine photoproducts at dipyrimidine sites. / Jen, J.; Mitchell, D. L.; Cunningham, R. P.; Smith, C. A.; Taylor, J. S.; Cleaver, J. E.

In: Photochemistry and Photobiology, Vol. 65, No. 2, 02.1997, p. 323-329.

Research output: Contribution to journalArticle

Jen, J, Mitchell, DL, Cunningham, RP, Smith, CA, Taylor, JS & Cleaver, JE 1997, 'Ultraviolet irradiation produces novel endonuclease III-sensitive cytosine photoproducts at dipyrimidine sites', Photochemistry and Photobiology, vol. 65, no. 2, pp. 323-329.
Jen J, Mitchell DL, Cunningham RP, Smith CA, Taylor JS, Cleaver JE. Ultraviolet irradiation produces novel endonuclease III-sensitive cytosine photoproducts at dipyrimidine sites. Photochemistry and Photobiology. 1997 Feb;65(2):323-329.
Jen, J. ; Mitchell, D. L. ; Cunningham, R. P. ; Smith, C. A. ; Taylor, J. S. ; Cleaver, J. E. / Ultraviolet irradiation produces novel endonuclease III-sensitive cytosine photoproducts at dipyrimidine sites. In: Photochemistry and Photobiology. 1997 ; Vol. 65, No. 2. pp. 323-329.
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