Ultraviolet irradiation of human skin causes functional vitamin A deficiency, preventable by all-trans retinoic acid pre-treatment

Zengquan Wang; Mohamed Boudjelal; Sewon Kang; John J. Voorhees; Gary J. Fisher

doi:10.1038/7417

Ultraviolet irradiation of human skin causes functional vitamin A deficiency, preventable by all-trans retinoic acid pre-treatment

Zengquan Wang, Mohamed Boudjelal, Sewon Kang, John J. Voorhees, Gary J. Fisher

Research output: Contribution to journal › Article › peer-review

99 Scopus citations

Abstract

We report here that ultraviolet irradiation substantially reduced the mRNA and protein of the two major nuclear retinoid receptors, RAR-γ and RXR- α, in human skin in vivo. Pre-treatment with retinoic acid mitigated this loss of nuclear retinoid receptors. Ultraviolet irradiation caused a near- total loss of retinoic acid induction of two RAR/RXR target genes, cellular retinoic acid binding protein-II and RA 4-hydroxylase, but did not affect 1,25-dihydroxyvitamin D₃ induction of the vitamin D receptor/RXR-regulated gene vitamin D 24-hydroxylase. In effect, ultraviolet irradiation causes a functional vitamin A deficiency that may have deleterious effects on skin function, contributing to skin photo-aging and carcinogenesis.

Original language	English (US)
Pages (from-to)	418-422
Number of pages	5
Journal	Nature medicine
Volume	5
Issue number	4
DOIs	https://doi.org/10.1038/7417
State	Published - Apr 1999
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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@article{25883221bcd44062802245280cd61494,

title = "Ultraviolet irradiation of human skin causes functional vitamin A deficiency, preventable by all-trans retinoic acid pre-treatment",

abstract = "We report here that ultraviolet irradiation substantially reduced the mRNA and protein of the two major nuclear retinoid receptors, RAR-γ and RXR- α, in human skin in vivo. Pre-treatment with retinoic acid mitigated this loss of nuclear retinoid receptors. Ultraviolet irradiation caused a near- total loss of retinoic acid induction of two RAR/RXR target genes, cellular retinoic acid binding protein-II and RA 4-hydroxylase, but did not affect 1,25-dihydroxyvitamin D3 induction of the vitamin D receptor/RXR-regulated gene vitamin D 24-hydroxylase. In effect, ultraviolet irradiation causes a functional vitamin A deficiency that may have deleterious effects on skin function, contributing to skin photo-aging and carcinogenesis.",

author = "Zengquan Wang and Mohamed Boudjelal and Sewon Kang and Voorhees, {John J.} and Fisher, {Gary J.}",

note = "Funding Information: Acknowledgments The authors thank C. Petersen and S. Rehbine for tissue procurement, J. Granger for technical assistance, L. VanGoor for graphics preparation, T. Hamilton for statistical analyses and A. Chapple for editorial assistance. We thank P. Chambon for RAR and RXR cDNA plasmids, M. Petkovich for human RA 4-hydroxylase cDNA, M. Haussler for human vitamin D 24-hydroxylase cDNA, and M.R. Uskokovic and P. Bollag at Hoffmann La Roche (Nutley, New Jersey) for 1,25-dihydroxyvitamin D3. This study was supported in part by the Babcock Endowment for Dermatological Research, the Dermatology Foundation (SK), and a grant from the Johnson & Johnson Corporation.",

year = "1999",

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doi = "10.1038/7417",

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T1 - Ultraviolet irradiation of human skin causes functional vitamin A deficiency, preventable by all-trans retinoic acid pre-treatment

AU - Wang, Zengquan

AU - Boudjelal, Mohamed

AU - Kang, Sewon

AU - Voorhees, John J.

AU - Fisher, Gary J.

N1 - Funding Information: Acknowledgments The authors thank C. Petersen and S. Rehbine for tissue procurement, J. Granger for technical assistance, L. VanGoor for graphics preparation, T. Hamilton for statistical analyses and A. Chapple for editorial assistance. We thank P. Chambon for RAR and RXR cDNA plasmids, M. Petkovich for human RA 4-hydroxylase cDNA, M. Haussler for human vitamin D 24-hydroxylase cDNA, and M.R. Uskokovic and P. Bollag at Hoffmann La Roche (Nutley, New Jersey) for 1,25-dihydroxyvitamin D3. This study was supported in part by the Babcock Endowment for Dermatological Research, the Dermatology Foundation (SK), and a grant from the Johnson & Johnson Corporation.

PY - 1999/4

Y1 - 1999/4

N2 - We report here that ultraviolet irradiation substantially reduced the mRNA and protein of the two major nuclear retinoid receptors, RAR-γ and RXR- α, in human skin in vivo. Pre-treatment with retinoic acid mitigated this loss of nuclear retinoid receptors. Ultraviolet irradiation caused a near- total loss of retinoic acid induction of two RAR/RXR target genes, cellular retinoic acid binding protein-II and RA 4-hydroxylase, but did not affect 1,25-dihydroxyvitamin D3 induction of the vitamin D receptor/RXR-regulated gene vitamin D 24-hydroxylase. In effect, ultraviolet irradiation causes a functional vitamin A deficiency that may have deleterious effects on skin function, contributing to skin photo-aging and carcinogenesis.

AB - We report here that ultraviolet irradiation substantially reduced the mRNA and protein of the two major nuclear retinoid receptors, RAR-γ and RXR- α, in human skin in vivo. Pre-treatment with retinoic acid mitigated this loss of nuclear retinoid receptors. Ultraviolet irradiation caused a near- total loss of retinoic acid induction of two RAR/RXR target genes, cellular retinoic acid binding protein-II and RA 4-hydroxylase, but did not affect 1,25-dihydroxyvitamin D3 induction of the vitamin D receptor/RXR-regulated gene vitamin D 24-hydroxylase. In effect, ultraviolet irradiation causes a functional vitamin A deficiency that may have deleterious effects on skin function, contributing to skin photo-aging and carcinogenesis.

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Ultraviolet irradiation of human skin causes functional vitamin A deficiency, preventable by all-trans retinoic acid pre-treatment

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