Ultraviolet irradiation induces CYR61/CCN1, a mediator of collagen homeostasis, through activation of transcription factor AP-1 in human skin fibroblasts

Taihao Quan, Zhaoping Qin, Yiru Xu, Tianyuan He, Sewon Kang, John J. Voorhees, Gary J. Fisher

Research output: Contribution to journalArticle

Abstract

UV irradiation from the sun elevates the production of collagen-degrading matrix metalloproteinases (MMPs) and reduces the production of new collagen. This imbalance of collagen homeostasis impairs the structure and function of the dermal collagenous extracellular matrix (ECM), thereby promoting premature skin aging (photoaging). We report here that aberrant dermal collagen homeostasis in UV-irradiated human skin is mediated in part by a CCN-family member, cysteine-rich protein-61 (CYR61/CCN1). CYR61 is significantly elevated in acutely UV-irradiated human skin in vivo, and UV-irradiated human skin fibroblasts. Knockdown of CYR61 significantly attenuates UV irradiation-induced inhibition of type-I procollagen and upregulation of MMP-1. Determination of CYR61 mRNA and protein indicates that the primary mechanism of CYR61 induction by UV irradiation is transcriptional. Analysis of CYR61 proximal promoter showed that a sequence conforming to the consensus binding site for transcription factor activator protein-1 (AP-1) is required for promoter activity. UV irradiation increased the binding of AP-1-family members c-Jun and c-Fos to this AP-1 site. Furthermore, functional blockade of c-Jun or knockdown of c-Jun significantly reduced the UV irradiation-induced activation of CYR61 promoter and CYR61 gene expression. These data show that CYR61 is transcriptionally regulated by UV irradiation through transcription factor AP-1, and mediates altered collagen homeostasis that occurs in response to UV irradiation in human skin fibroblasts.

Original languageEnglish (US)
Pages (from-to)1697-1706
Number of pages10
JournalJournal of Investigative Dermatology
Volume130
Issue number6
DOIs
StatePublished - Jun 2010
Externally publishedYes

Fingerprint

Transcription Factor AP-1
Fibroblasts
Skin
Homeostasis
Transcription Factors
Collagen
Chemical activation
Irradiation
Cysteine-Rich Protein 61
Premature Aging
Skin Aging
Matrix Metalloproteinase 1
Solar System
Collagen Type I
Matrix Metalloproteinases
Extracellular Matrix
Gene expression
Carrier Proteins
Sun
Up-Regulation

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Ultraviolet irradiation induces CYR61/CCN1, a mediator of collagen homeostasis, through activation of transcription factor AP-1 in human skin fibroblasts. / Quan, Taihao; Qin, Zhaoping; Xu, Yiru; He, Tianyuan; Kang, Sewon; Voorhees, John J.; Fisher, Gary J.

In: Journal of Investigative Dermatology, Vol. 130, No. 6, 06.2010, p. 1697-1706.

Research output: Contribution to journalArticle

Quan, Taihao ; Qin, Zhaoping ; Xu, Yiru ; He, Tianyuan ; Kang, Sewon ; Voorhees, John J. ; Fisher, Gary J. / Ultraviolet irradiation induces CYR61/CCN1, a mediator of collagen homeostasis, through activation of transcription factor AP-1 in human skin fibroblasts. In: Journal of Investigative Dermatology. 2010 ; Vol. 130, No. 6. pp. 1697-1706.
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