Ultrastructural immunocytochemical localization of μ opioid receptors and Leu5-enkephalin in the patch compartment of the rat caudate-putamen nucleus

Hong Wang, Akiyoshi Moriwaki, Jia Bei Wang, George R. Uhl, Virginia M. Pickel

Research output: Contribution to journalArticle

Abstract

To delineate the cellular sites for the motor effects of opiates acting at the μ opioid receptor (MOR) in the rat caudate-putamen nucleus, we examined the ultrastructural immunogold and immunoperoxidase labeling of an antipeptide antiserum specific for the MOR. We also combined these labeling methods to examine the subcellular relationship between the MOR and the endogenous opioid peptide, Leu5-enkephalin (LE). By light microscopy, MOR- labeling was seen in a heterogeneous patchy distribution. Electron microscopic analysis of these patches showed that more than 80% of the total neuronal profiles (n = 1,586) containing MOR-like immunoreactivity (MOR-IR) were dendrites and dendritic spines. The remaining labeled profiles included a few perikarya and many axon terminals. MOR-IR was predominantly localized to extrasynaptic plasma membranes of dendrites, and to both synaptic vesicles and plasma membranes in terminals. Ten percent of the total MOR-labeled terminals (n = 272) formed asymmetric synapses with unlabeled or MOR-labeled dendritic spines. Terminals containing LE-IR formed synapses, in almost equal proportions, on MOR-labeled dendrites and dendritic spines, while over 80% of the unlabeled terminals formed synapses on MOR-labeled dendritic spines. Moreover, colocalization of MOR- and LE-IR was often seen in both dendrites and terminals. These results indicate that in patch compartments of the caudate-putamen nucleus, the MOR is mainly involved in extrasynaptic modulation of spiny neurons, including those that contain LE. In addition, the findings provide a cellular basis for presynaptic opioid modulation of neurotransmitter release through MOR located on axon terminals.

Original languageEnglish (US)
Pages (from-to)659-674
Number of pages16
JournalJournal of Comparative Neurology
Volume375
Issue number4
DOIs
StatePublished - Nov 25 1996

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Dendritic Spines
Enkephalins
Caudate Nucleus
Putamen
Opioid Receptors
Dendrites
Synapses
Presynaptic Terminals
Opiate Alkaloids
Cell Membrane
Synaptic Membranes
Opioid Peptides
Synaptic Vesicles
Opioid Analgesics
Neurotransmitter Agents
Immune Sera
Microscopy
Electrons
Neurons
Light

Keywords

  • electron microscopy
  • endogenous opioid
  • extrasynaptic site
  • morphine
  • stereotyped behavior

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ultrastructural immunocytochemical localization of μ opioid receptors and Leu5-enkephalin in the patch compartment of the rat caudate-putamen nucleus. / Wang, Hong; Moriwaki, Akiyoshi; Wang, Jia Bei; Uhl, George R.; Pickel, Virginia M.

In: Journal of Comparative Neurology, Vol. 375, No. 4, 25.11.1996, p. 659-674.

Research output: Contribution to journalArticle

Wang, Hong ; Moriwaki, Akiyoshi ; Wang, Jia Bei ; Uhl, George R. ; Pickel, Virginia M. / Ultrastructural immunocytochemical localization of μ opioid receptors and Leu5-enkephalin in the patch compartment of the rat caudate-putamen nucleus. In: Journal of Comparative Neurology. 1996 ; Vol. 375, No. 4. pp. 659-674.
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