Abstract
Human mast cells developed in vitro when cord blood mononuclear cells were cocultured for 3 months with 3T3 embryonic mouse skin fibroblasts. The metachromatic cells that arose in these cultures contained histamine, a functional Fc(ε) receptor and granule proteases (tryptase, chymase), and they were definitively identified by the ultrastructural demonstration of crystal granules. We present a detailed ultrastructural analysis of this newly available system for the reliable development of human mast cells in vitro and provide criteria for definitive identification of the mast cell and basophil lineages in humans.
Original language | English (US) |
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Pages (from-to) | 557-569 |
Number of pages | 13 |
Journal | Journal of Leukocyte Biology |
Volume | 51 |
Issue number | 6 |
DOIs | |
State | Published - 1992 |
Externally published | Yes |
Keywords
- basophil
- endothelial cell
- eosinophil
- interleukin 3
- macrophage
- secretory granules
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Cell Biology