Ultrastructural analysis of myoblast fusion in Drosophila.

Shiliang Zhang, Elizabeth H. Chen

Research output: Contribution to journalArticle

Abstract

Myoblast fusion in Drosophila has become a powerful genetic system with which to unravel the mechanisms underlying cell fusion. The identification of important components of myoblast fusion by genetic analysis has led to a molecular pathway toward our understanding of this cellular process. In addition to the application of immunohistochemistry and live imaging techniques to visualize myoblast fusion at the light microscopic level, ultrastructural analysis using electron microscopy remains an indispensable tool to reveal fusion intermediates and specific membrane events at sites of fusion. In this chapter, we describe conventional chemical fixation and high-pressure freezing/freeze substitution methods for visualizing fusion intermediates during Drosophila myoblast fusion. Furthermore, we describe an immunoelectron microscopic method for localizing specific proteins relative to the fusion apparatus.

Original languageEnglish (US)
Pages (from-to)275-297
Number of pages23
JournalMethods in molecular biology (Clifton, N.J.)
Volume475
StatePublished - 2008

Fingerprint

Myoblasts
Drosophila
Freeze Substitution
Cell Fusion
Freezing
Electron Microscopy
Immunohistochemistry
Light
Pressure
Membranes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ultrastructural analysis of myoblast fusion in Drosophila. / Zhang, Shiliang; Chen, Elizabeth H.

In: Methods in molecular biology (Clifton, N.J.), Vol. 475, 2008, p. 275-297.

Research output: Contribution to journalArticle

Zhang, Shiliang ; Chen, Elizabeth H. / Ultrastructural analysis of myoblast fusion in Drosophila. In: Methods in molecular biology (Clifton, N.J.). 2008 ; Vol. 475. pp. 275-297.
@article{603a258acc3f49dd8958608d9073cbe5,
title = "Ultrastructural analysis of myoblast fusion in Drosophila.",
abstract = "Myoblast fusion in Drosophila has become a powerful genetic system with which to unravel the mechanisms underlying cell fusion. The identification of important components of myoblast fusion by genetic analysis has led to a molecular pathway toward our understanding of this cellular process. In addition to the application of immunohistochemistry and live imaging techniques to visualize myoblast fusion at the light microscopic level, ultrastructural analysis using electron microscopy remains an indispensable tool to reveal fusion intermediates and specific membrane events at sites of fusion. In this chapter, we describe conventional chemical fixation and high-pressure freezing/freeze substitution methods for visualizing fusion intermediates during Drosophila myoblast fusion. Furthermore, we describe an immunoelectron microscopic method for localizing specific proteins relative to the fusion apparatus.",
author = "Shiliang Zhang and Chen, {Elizabeth H.}",
year = "2008",
language = "English (US)",
volume = "475",
pages = "275--297",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Ultrastructural analysis of myoblast fusion in Drosophila.

AU - Zhang, Shiliang

AU - Chen, Elizabeth H.

PY - 2008

Y1 - 2008

N2 - Myoblast fusion in Drosophila has become a powerful genetic system with which to unravel the mechanisms underlying cell fusion. The identification of important components of myoblast fusion by genetic analysis has led to a molecular pathway toward our understanding of this cellular process. In addition to the application of immunohistochemistry and live imaging techniques to visualize myoblast fusion at the light microscopic level, ultrastructural analysis using electron microscopy remains an indispensable tool to reveal fusion intermediates and specific membrane events at sites of fusion. In this chapter, we describe conventional chemical fixation and high-pressure freezing/freeze substitution methods for visualizing fusion intermediates during Drosophila myoblast fusion. Furthermore, we describe an immunoelectron microscopic method for localizing specific proteins relative to the fusion apparatus.

AB - Myoblast fusion in Drosophila has become a powerful genetic system with which to unravel the mechanisms underlying cell fusion. The identification of important components of myoblast fusion by genetic analysis has led to a molecular pathway toward our understanding of this cellular process. In addition to the application of immunohistochemistry and live imaging techniques to visualize myoblast fusion at the light microscopic level, ultrastructural analysis using electron microscopy remains an indispensable tool to reveal fusion intermediates and specific membrane events at sites of fusion. In this chapter, we describe conventional chemical fixation and high-pressure freezing/freeze substitution methods for visualizing fusion intermediates during Drosophila myoblast fusion. Furthermore, we describe an immunoelectron microscopic method for localizing specific proteins relative to the fusion apparatus.

UR - http://www.scopus.com/inward/record.url?scp=58149149378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149149378&partnerID=8YFLogxK

M3 - Article

C2 - 18979250

VL - 475

SP - 275

EP - 297

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -