Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus

Michimasa Suzuki, Laure Bachelet-Violette, François Rouzet, Anne Beilvert, Gwennhael Autret, Murielle Maire, Christine Menager, Liliane Louedec, Christine Choqueux, Pierre Saboural, Oualid Haddad, Cédric Chauvierre, Frédéric Chaubet, Jean Baptiste Michel, Jean Michel Serfaty, Didier Letourneur

Research output: Contribution to journalArticle

Abstract

Aim: We have designed ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles associated with fucoidan (USPOI-FUCO), a natural sulfated polysaccharide with high affinity for activated platelets, to visualize by MRI arterial thrombi. Materials & methods: USPIOs were prepared and sizes, zeta-potentials and relaxivities were measured. Elastase perfusion in the infrarenal aorta of Wistar rats induced intraluminal thrombus. They were scanned on 4.7 T MRI before and after injection of USPIO-FUCO or USPIO coated with anionic dextran. Results: Surface plasmon resonance evidenced that fucoidan and USPIO-FUCO bind in vitro to immobilized P-selectin. All intraluminal hyposignals detected by MRI after injection of USPIO-FUCO on animals (13 out of 13) were correlated by histology with thrombi, whereas none could be identified with control USPIOs (0 out of 7). No signal was seen in absence of thrombus. Thrombi by MRI were correlated with P-selectin immunostaining and USPIO detection by electron microscopy. Conclusion: In vivo thrombi can thus be evidenced by MRI with USPIO-FUCO.

Original languageEnglish (US)
Pages (from-to)73-87
Number of pages15
JournalNanomedicine
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Iron oxides
Nanoparticles
Magnetic resonance imaging
iron oxide
Thrombosis
animal
P-Selectin
Histology
Dextran
histology
Surface plasmon resonance
Zeta potential
Polysaccharides
Platelets
fucoidan
ferumoxtran-10
nanoparticle
electron microscopy
polysaccharide
Injections

Keywords

  • aneurysm
  • iron oxide nanoparticle
  • MRI
  • P-selectin
  • thrombus

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development
  • Medicine(all)

Cite this

Suzuki, M., Bachelet-Violette, L., Rouzet, F., Beilvert, A., Autret, G., Maire, M., ... Letourneur, D. (2015). Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus. Nanomedicine, 10(1), 73-87. https://doi.org/10.2217/nnm.14.51

Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus. / Suzuki, Michimasa; Bachelet-Violette, Laure; Rouzet, François; Beilvert, Anne; Autret, Gwennhael; Maire, Murielle; Menager, Christine; Louedec, Liliane; Choqueux, Christine; Saboural, Pierre; Haddad, Oualid; Chauvierre, Cédric; Chaubet, Frédéric; Michel, Jean Baptiste; Serfaty, Jean Michel; Letourneur, Didier.

In: Nanomedicine, Vol. 10, No. 1, 01.01.2015, p. 73-87.

Research output: Contribution to journalArticle

Suzuki, M, Bachelet-Violette, L, Rouzet, F, Beilvert, A, Autret, G, Maire, M, Menager, C, Louedec, L, Choqueux, C, Saboural, P, Haddad, O, Chauvierre, C, Chaubet, F, Michel, JB, Serfaty, JM & Letourneur, D 2015, 'Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus', Nanomedicine, vol. 10, no. 1, pp. 73-87. https://doi.org/10.2217/nnm.14.51
Suzuki, Michimasa ; Bachelet-Violette, Laure ; Rouzet, François ; Beilvert, Anne ; Autret, Gwennhael ; Maire, Murielle ; Menager, Christine ; Louedec, Liliane ; Choqueux, Christine ; Saboural, Pierre ; Haddad, Oualid ; Chauvierre, Cédric ; Chaubet, Frédéric ; Michel, Jean Baptiste ; Serfaty, Jean Michel ; Letourneur, Didier. / Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus. In: Nanomedicine. 2015 ; Vol. 10, No. 1. pp. 73-87.
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AU - Autret, Gwennhael

AU - Maire, Murielle

AU - Menager, Christine

AU - Louedec, Liliane

AU - Choqueux, Christine

AU - Saboural, Pierre

AU - Haddad, Oualid

AU - Chauvierre, Cédric

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