Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus

Michimasa Suzuki, Laure Bachelet-Violette, François Rouzet, Anne Beilvert, Gwennhael Autret, Murielle Maire, Christine Menager, Liliane Louedec, Christine Choqueux, Pierre Saboural, Oualid Haddad, Cédric Chauvierre, Frédéric Chaubet, Jean Baptiste Michel, Jean Michel Serfaty, Didier Letourneur

Research output: Contribution to journalArticlepeer-review


Aim: We have designed ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles associated with fucoidan (USPOI-FUCO), a natural sulfated polysaccharide with high affinity for activated platelets, to visualize by MRI arterial thrombi. Materials & methods: USPIOs were prepared and sizes, zeta-potentials and relaxivities were measured. Elastase perfusion in the infrarenal aorta of Wistar rats induced intraluminal thrombus. They were scanned on 4.7 T MRI before and after injection of USPIO-FUCO or USPIO coated with anionic dextran. Results: Surface plasmon resonance evidenced that fucoidan and USPIO-FUCO bind in vitro to immobilized P-selectin. All intraluminal hyposignals detected by MRI after injection of USPIO-FUCO on animals (13 out of 13) were correlated by histology with thrombi, whereas none could be identified with control USPIOs (0 out of 7). No signal was seen in absence of thrombus. Thrombi by MRI were correlated with P-selectin immunostaining and USPIO detection by electron microscopy. Conclusion: In vivo thrombi can thus be evidenced by MRI with USPIO-FUCO.

Original languageEnglish (US)
Pages (from-to)73-87
Number of pages15
Issue number1
StatePublished - Jan 1 2015
Externally publishedYes


  • aneurysm
  • iron oxide nanoparticle
  • MRI
  • P-selectin
  • thrombus

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development
  • Medicine(all)


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