Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus

Michimasa Suzuki; Laure Bachelet-Violette; François Rouzet; Anne Beilvert; Gwennhael Autret; Murielle Maire; Christine Menager; Liliane Louedec; Christine Choqueux; Pierre Saboural; Oualid Haddad; Cédric Chauvierre; Frédéric Chaubet; Jean Baptiste Michel; Jean Michel Serfaty; Didier Letourneur

doi:10.2217/nnm.14.51

Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus

Michimasa Suzuki, Laure Bachelet-Violette, François Rouzet, Anne Beilvert, Gwennhael Autret, Murielle Maire, Christine Menager, Liliane Louedec, Christine Choqueux, Pierre Saboural, Oualid Haddad, Cédric Chauvierre, Frédéric Chaubet, Jean Baptiste Michel, Jean Michel Serfaty, Didier Letourneur

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Aim: We have designed ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles associated with fucoidan (USPOI-FUCO), a natural sulfated polysaccharide with high affinity for activated platelets, to visualize by MRI arterial thrombi. Materials & methods: USPIOs were prepared and sizes, zeta-potentials and relaxivities were measured. Elastase perfusion in the infrarenal aorta of Wistar rats induced intraluminal thrombus. They were scanned on 4.7 T MRI before and after injection of USPIO-FUCO or USPIO coated with anionic dextran. Results: Surface plasmon resonance evidenced that fucoidan and USPIO-FUCO bind in vitro to immobilized P-selectin. All intraluminal hyposignals detected by MRI after injection of USPIO-FUCO on animals (13 out of 13) were correlated by histology with thrombi, whereas none could be identified with control USPIOs (0 out of 7). No signal was seen in absence of thrombus. Thrombi by MRI were correlated with P-selectin immunostaining and USPIO detection by electron microscopy. Conclusion: In vivo thrombi can thus be evidenced by MRI with USPIO-FUCO.

Original language	English (US)
Pages (from-to)	73-87
Number of pages	15
Journal	Nanomedicine
Volume	10
Issue number	1
DOIs	https://doi.org/10.2217/nnm.14.51
State	Published - Jan 1 2015
Externally published	Yes

Keywords

aneurysm
iron oxide nanoparticle
MRI
P-selectin
thrombus

ASJC Scopus subject areas

General Materials Science
Bioengineering
Biomedical Engineering
Medicine (miscellaneous)
Development
General Medicine

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Suzuki, M., Bachelet-Violette, L., Rouzet, F., Beilvert, A., Autret, G., Maire, M., Menager, C., Louedec, L., Choqueux, C., Saboural, P., Haddad, O., Chauvierre, C., Chaubet, F., Michel, J. B., Serfaty, J. M., & Letourneur, D. (2015). Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus. Nanomedicine, 10(1), 73-87. https://doi.org/10.2217/nnm.14.51

Suzuki, M, Bachelet-Violette, L, Rouzet, F, Beilvert, A, Autret, G, Maire, M, Menager, C, Louedec, L, Choqueux, C, Saboural, P, Haddad, O, Chauvierre, C, Chaubet, F, Michel, JB, Serfaty, JM & Letourneur, D 2015, 'Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus', Nanomedicine, vol. 10, no. 1, pp. 73-87. https://doi.org/10.2217/nnm.14.51

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abstract = "Aim: We have designed ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles associated with fucoidan (USPOI-FUCO), a natural sulfated polysaccharide with high affinity for activated platelets, to visualize by MRI arterial thrombi. Materials & methods: USPIOs were prepared and sizes, zeta-potentials and relaxivities were measured. Elastase perfusion in the infrarenal aorta of Wistar rats induced intraluminal thrombus. They were scanned on 4.7 T MRI before and after injection of USPIO-FUCO or USPIO coated with anionic dextran. Results: Surface plasmon resonance evidenced that fucoidan and USPIO-FUCO bind in vitro to immobilized P-selectin. All intraluminal hyposignals detected by MRI after injection of USPIO-FUCO on animals (13 out of 13) were correlated by histology with thrombi, whereas none could be identified with control USPIOs (0 out of 7). No signal was seen in absence of thrombus. Thrombi by MRI were correlated with P-selectin immunostaining and USPIO detection by electron microscopy. Conclusion: In vivo thrombi can thus be evidenced by MRI with USPIO-FUCO.",

keywords = "aneurysm, iron oxide nanoparticle, MRI, P-selectin, thrombus",

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AU - Suzuki, Michimasa

AU - Bachelet-Violette, Laure

AU - Rouzet, François

AU - Beilvert, Anne

AU - Autret, Gwennhael

AU - Maire, Murielle

AU - Menager, Christine

AU - Louedec, Liliane

AU - Choqueux, Christine

AU - Saboural, Pierre

AU - Haddad, Oualid

AU - Chauvierre, Cédric

AU - Chaubet, Frédéric

AU - Michel, Jean Baptiste

AU - Serfaty, Jean Michel

AU - Letourneur, Didier

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Aim: We have designed ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles associated with fucoidan (USPOI-FUCO), a natural sulfated polysaccharide with high affinity for activated platelets, to visualize by MRI arterial thrombi. Materials & methods: USPIOs were prepared and sizes, zeta-potentials and relaxivities were measured. Elastase perfusion in the infrarenal aorta of Wistar rats induced intraluminal thrombus. They were scanned on 4.7 T MRI before and after injection of USPIO-FUCO or USPIO coated with anionic dextran. Results: Surface plasmon resonance evidenced that fucoidan and USPIO-FUCO bind in vitro to immobilized P-selectin. All intraluminal hyposignals detected by MRI after injection of USPIO-FUCO on animals (13 out of 13) were correlated by histology with thrombi, whereas none could be identified with control USPIOs (0 out of 7). No signal was seen in absence of thrombus. Thrombi by MRI were correlated with P-selectin immunostaining and USPIO detection by electron microscopy. Conclusion: In vivo thrombi can thus be evidenced by MRI with USPIO-FUCO.

AB - Aim: We have designed ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles associated with fucoidan (USPOI-FUCO), a natural sulfated polysaccharide with high affinity for activated platelets, to visualize by MRI arterial thrombi. Materials & methods: USPIOs were prepared and sizes, zeta-potentials and relaxivities were measured. Elastase perfusion in the infrarenal aorta of Wistar rats induced intraluminal thrombus. They were scanned on 4.7 T MRI before and after injection of USPIO-FUCO or USPIO coated with anionic dextran. Results: Surface plasmon resonance evidenced that fucoidan and USPIO-FUCO bind in vitro to immobilized P-selectin. All intraluminal hyposignals detected by MRI after injection of USPIO-FUCO on animals (13 out of 13) were correlated by histology with thrombi, whereas none could be identified with control USPIOs (0 out of 7). No signal was seen in absence of thrombus. Thrombi by MRI were correlated with P-selectin immunostaining and USPIO detection by electron microscopy. Conclusion: In vivo thrombi can thus be evidenced by MRI with USPIO-FUCO.

KW - aneurysm

KW - iron oxide nanoparticle

KW - MRI

KW - P-selectin

KW - thrombus

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Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus

Abstract

Keywords

ASJC Scopus subject areas

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