Ultrasensitive glycogen synthesis in Cyanobacteria

Diego F. Gómez Casati, Miguel A. Aon, Alberto A. Iglesias

Research output: Contribution to journalArticle

Abstract

Cyanobacter ADPglucose pyrophosphorylase exhibits a ultrasensitive response in activity towards its allosteric effector 3-phosphoglycerate, elicited by orthophosphate and polyethyleneglycol-induced molecular crowding. The ultrasensitive response was observed either when the enzyme operates in the zero or first order region for its physiological substrates. The ultrasensitivity exhibited maximal amplification factors of 15-19-fold with respect to 1% of the maximal system velocity. Only a 2.4-3.8-fold increase in 3PGA concentration was necessary to augment the flux from 10% to 90% through AGPase as compared with 200-fold required for the control. The results are discussed in terms of finely tuned regulatory mechanisms of polysaccharide synthesis in oxygenic photosynthetic organisms. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)117-121
Number of pages5
JournalFEBS Letters
Volume446
Issue number1
DOIs
StatePublished - Mar 5 1999

Keywords

  • ADPglucose pyrophosphorylase
  • Anabaena PCC 7120
  • Enzyme regulation
  • Glycogen and starch biosynthesis
  • Molecular crowding

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Gómez Casati, D. F., Aon, M. A., & Iglesias, A. A. (1999). Ultrasensitive glycogen synthesis in Cyanobacteria. FEBS Letters, 446(1), 117-121. https://doi.org/10.1016/S0014-5793(99)00201-X