Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria

Diego F. Gómez-Casati, Sonia Cortassa, Miguel A. Aon, Alberto A. Iglesias

Research output: Contribution to journalArticle

Abstract

The glycogen synthetic pathway operates ultrasensitively as a function of the ADPglucose pyrophosphorylase (ADPGlcPPase) allosteric effectors, 3-phosphoglycerate and Pi, in permeabilized cells of the cyanobacterium Anabaena PCC 7120. In vitro data previously showed that the ultrasensitive behavior of ADPGlcPPase depends upon cross-talk between the two allosteric effectors, the enzyme's response being additionally modulated by molecular crowding [D.F. Gómez Casatiet al. (2000) Biochem J 350:139-147]. In the present work we show, experimentally and with a mathematical model, that α-1,4-glucan synthesis is also ultrasensitive in cells due to the propagation of the switch-like behavior of ADPGlcPPase to the synthetic pathway. Amplifications of up to 20-fold in storage-polysaccharide synthesis can be achieved with a modest 6.7-fold increase in 3-phosphoglycerate in the presence of 5 mM Pi in contrast to the 30-fold necessary in its absence. This is the first time that this phenomenon has been reported to occur in the glycogen synthetic pathway of a photosynthetic prokaryote. The implications of the results for plant cell physiology during light-dark transitions are discussed.

Original languageEnglish (US)
Pages (from-to)969-975
Number of pages7
JournalPlanta
Volume216
Issue number6
StatePublished - Apr 1 2003
Externally publishedYes

Fingerprint

Glucose-1-Phosphate Adenylyltransferase
Cyanobacteria
Polysaccharides
phosphoglyceric acids
polysaccharides
Glycogen
glycogen
synthesis
Plant Physiological Phenomena
Anabaena
cell physiology
Cell Physiological Phenomena
Plant Cells
glucans
prokaryotic cells
Theoretical Models
mathematical models
cells
Light
Enzymes

Keywords

  • ADPglucose pyrophosphorylase
  • Anabaena
  • Glycogen/starch synthesis
  • Molecular crowding
  • Permeabilized cyanobacteria
  • Ultrasensitivity (of enzymes)

ASJC Scopus subject areas

  • Plant Science

Cite this

Gómez-Casati, D. F., Cortassa, S., Aon, M. A., & Iglesias, A. A. (2003). Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria. Planta, 216(6), 969-975.

Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria. / Gómez-Casati, Diego F.; Cortassa, Sonia; Aon, Miguel A.; Iglesias, Alberto A.

In: Planta, Vol. 216, No. 6, 01.04.2003, p. 969-975.

Research output: Contribution to journalArticle

Gómez-Casati, DF, Cortassa, S, Aon, MA & Iglesias, AA 2003, 'Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria', Planta, vol. 216, no. 6, pp. 969-975.
Gómez-Casati DF, Cortassa S, Aon MA, Iglesias AA. Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria. Planta. 2003 Apr 1;216(6):969-975.
Gómez-Casati, Diego F. ; Cortassa, Sonia ; Aon, Miguel A. ; Iglesias, Alberto A. / Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria. In: Planta. 2003 ; Vol. 216, No. 6. pp. 969-975.
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