Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions

Shigeki Watanabe, Qiang Liu, M. Wayne Davis, Gunther Hollopeter, Nikita Thomas, Nels B. Jorgensen, Erik M. Jorgensen

Research output: Contribution to journalArticlepeer-review

Abstract

Synaptic vesicles can be released at extremely high rates, which places an extraordinary demand on the recycling machinery. Previous ultrastructural studies of vesicle recycling were conducted in dissected preparations using an intense stimulation to maximize the probability of release. Here, a single light stimulus was applied to motor neurons in intact Caenorhabditis elegans nematodes expressing channelrhodopsin, and the animals rapidly frozen. We found that docked vesicles fuse along a broad active zone in response to a single stimulus, and are replenished with a time constant of about 2 s. Endocytosis occurs within 50 ms adjacent to the dense projection and after 1 s adjacent to adherens junctions. These studies suggest that synaptic vesicle endocytosis may occur on a millisecond time scale following a single physiological stimulus in the intact nervous system and is unlikely to conform to current models of endocytosis.

Original languageEnglish (US)
Article numbere00723
JournaleLife
Volume2013
Issue number2
DOIs
StatePublished - Sep 3 2013
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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