TY - JOUR
T1 - Ulcerative Colitis
T2 - Novel Epithelial Insights Provided by Single Cell RNA Sequencing
AU - Serigado, Joao M.
AU - Foulke-Abel, Jennifer
AU - Hines, William C.
AU - Hanson, Joshua A.
AU - In, Julie
AU - Kovbasnjuk, Olga
N1 - Funding Information:
This research was supported by NIH grants U01 DK103168, P01 AI125181 to OK, JI, and JF-A, K01 DK113043 to JF-A, and K01 DK106323 to JI, and by University of New Mexico Department of Internal Medicine start-up funds to JI and OK.
Publisher Copyright:
Copyright © 2022 Serigado, Foulke-Abel, Hines, Hanson, In and Kovbasnjuk.
PY - 2022/4/20
Y1 - 2022/4/20
N2 - Ulcerative Colitis (UC) is a chronic inflammatory disease of the intestinal tract for which a definitive etiology is yet unknown. Both genetic and environmental factors have been implicated in the development of UC. Recently, single cell RNA sequencing (scRNA-seq) technology revealed cell subpopulations contributing to the pathogenesis of UC and brought new insight into the pathways that connect genome to pathology. This review describes key scRNA-seq findings in two major studies by Broad Institute and University of Oxford, investigating the transcriptomic landscape of epithelial cells in UC. We focus on five major findings: (1) the identification of BEST4 + cells, (2) colonic microfold (M) cells, (3) detailed comparison of the transcriptomes of goblet cells, and (4) colonocytes and (5) stem cells in health and disease. In analyzing the two studies, we identify the commonalities and differences in methodologies, results, and conclusions, offering possible explanations, and validated several cell cluster markers. In systematizing the results, we hope to offer a framework that the broad scientific GI community and GI clinicians can use to replicate or corroborate the extensive new findings that RNA-seq offers.
AB - Ulcerative Colitis (UC) is a chronic inflammatory disease of the intestinal tract for which a definitive etiology is yet unknown. Both genetic and environmental factors have been implicated in the development of UC. Recently, single cell RNA sequencing (scRNA-seq) technology revealed cell subpopulations contributing to the pathogenesis of UC and brought new insight into the pathways that connect genome to pathology. This review describes key scRNA-seq findings in two major studies by Broad Institute and University of Oxford, investigating the transcriptomic landscape of epithelial cells in UC. We focus on five major findings: (1) the identification of BEST4 + cells, (2) colonic microfold (M) cells, (3) detailed comparison of the transcriptomes of goblet cells, and (4) colonocytes and (5) stem cells in health and disease. In analyzing the two studies, we identify the commonalities and differences in methodologies, results, and conclusions, offering possible explanations, and validated several cell cluster markers. In systematizing the results, we hope to offer a framework that the broad scientific GI community and GI clinicians can use to replicate or corroborate the extensive new findings that RNA-seq offers.
KW - Ulcerative Colitis
KW - colonic microfold cells
KW - goblet cells
KW - intestinal epithelium
KW - single cell RNA sequencing
KW - stem cells
UR - http://www.scopus.com/inward/record.url?scp=85129372745&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85129372745&partnerID=8YFLogxK
U2 - 10.3389/fmed.2022.868508
DO - 10.3389/fmed.2022.868508
M3 - Review article
C2 - 35530046
AN - SCOPUS:85129372745
SN - 2296-858X
VL - 9
JO - Frontiers in Medicine
JF - Frontiers in Medicine
M1 - 868508
ER -