Ubiqutination via K27 and K29 chains signals aggregation and neuronal protection of LRRK2 by WSB1

Frederick Nucifora, Leslie Nucifora, Chee Hoe Ng, Nicolas Arbez, Yajuan Guo, Elaine Roby, Vered Shani, Simone Engelender, Dong Wei, Xiao Fang Wang, Tianxia Li, Darren J. Moore, Olga Pletnikova, Juan C Troncoso, Akira Sawa, Ted M Dawson, Wanli Smith, Kah Leong Lim, Christopher A Ross

Research output: Contribution to journalArticle

Abstract

A common genetic form of Parkinson's disease (PD) is caused by mutations in LRRK2. We identify WSB1 as a LRRK2 interacting protein. WSB1 ubiquitinates LRRK2 through K27 and K29 linkage chains, leading to LRRK2 aggregation and neuronal protection in primary neurons and a Drosophila model of G2019S LRRK2. Knocking down endogenous WSB1 exacerbates mutant LRRK2 neuronal toxicity in neurons and the Drosophila model, indicating a role for endogenous WSB1 in modulating LRRK2 cell toxicity. WSB1 is in Lewy bodies in human PD post-mortem tissue. These data demonstrate a role for WSB1 in mutant LRRK2 pathogenesis, and suggest involvement in Lewy body pathology in sporadic PD. Our data indicate a role in PD for ubiquitin K27 and K29 linkages, and suggest that ubiquitination may be a signal for aggregation and neuronal protection in PD, which may be relevant for other neurodegenerative disorders. Finally, our study identifies a novel therapeutic target for PD.

Original languageEnglish (US)
Article number11792
JournalNature Communications
Volume7
DOIs
StatePublished - Jun 7 2016

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Parkinson disease
Parkinson Disease
Agglomeration
Drosophila
Lewy Bodies
neurons
linkages
toxicity
Neurons
Toxicity
pathogenesis
Ubiquitination
pathology
Pathology
mutations
Ubiquitin
Neurodegenerative Diseases
Neuroprotection
disorders
Tissue

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Ubiqutination via K27 and K29 chains signals aggregation and neuronal protection of LRRK2 by WSB1. / Nucifora, Frederick; Nucifora, Leslie; Ng, Chee Hoe; Arbez, Nicolas; Guo, Yajuan; Roby, Elaine; Shani, Vered; Engelender, Simone; Wei, Dong; Wang, Xiao Fang; Li, Tianxia; Moore, Darren J.; Pletnikova, Olga; Troncoso, Juan C; Sawa, Akira; Dawson, Ted M; Smith, Wanli; Lim, Kah Leong; Ross, Christopher A.

In: Nature Communications, Vol. 7, 11792, 07.06.2016.

Research output: Contribution to journalArticle

Nucifora, Frederick ; Nucifora, Leslie ; Ng, Chee Hoe ; Arbez, Nicolas ; Guo, Yajuan ; Roby, Elaine ; Shani, Vered ; Engelender, Simone ; Wei, Dong ; Wang, Xiao Fang ; Li, Tianxia ; Moore, Darren J. ; Pletnikova, Olga ; Troncoso, Juan C ; Sawa, Akira ; Dawson, Ted M ; Smith, Wanli ; Lim, Kah Leong ; Ross, Christopher A. / Ubiqutination via K27 and K29 chains signals aggregation and neuronal protection of LRRK2 by WSB1. In: Nature Communications. 2016 ; Vol. 7.
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abstract = "A common genetic form of Parkinson's disease (PD) is caused by mutations in LRRK2. We identify WSB1 as a LRRK2 interacting protein. WSB1 ubiquitinates LRRK2 through K27 and K29 linkage chains, leading to LRRK2 aggregation and neuronal protection in primary neurons and a Drosophila model of G2019S LRRK2. Knocking down endogenous WSB1 exacerbates mutant LRRK2 neuronal toxicity in neurons and the Drosophila model, indicating a role for endogenous WSB1 in modulating LRRK2 cell toxicity. WSB1 is in Lewy bodies in human PD post-mortem tissue. These data demonstrate a role for WSB1 in mutant LRRK2 pathogenesis, and suggest involvement in Lewy body pathology in sporadic PD. Our data indicate a role in PD for ubiquitin K27 and K29 linkages, and suggest that ubiquitination may be a signal for aggregation and neuronal protection in PD, which may be relevant for other neurodegenerative disorders. Finally, our study identifies a novel therapeutic target for PD.",
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AU - Roby, Elaine

AU - Shani, Vered

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AU - Troncoso, Juan C

AU - Sawa, Akira

AU - Dawson, Ted M

AU - Smith, Wanli

AU - Lim, Kah Leong

AU - Ross, Christopher A

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