Ubiquity of the repetitive epitope of the CS protein in different isolates of human malaria parasites

Fidel P Zavala, A. Masuda, P. M. Graves, V. Nussenzweig, R. S. Nussenzweig

Research output: Contribution to journalArticle

Abstract

Sporozoites of the human malaria Plasmodium falciparum and Plasmodium vivax obtained from a large number of endemic areas were screened with species-specific monoclonal antibodies that recognize the repeated epitopes of the respective circumsporozoite (CS) proteins. By using a two-site immunoradiometric assay, it was determined that all the parasite isolates of a given species react with a single monoclonal antibody, indicating the presence of a common repeated epitope. Polyacrylamide gel electrophoresis, followed by Western blot, showed that the CS proteins of the various isolates differed in their apparent m.w.

Original languageEnglish (US)
Pages (from-to)2790-2793
Number of pages4
JournalJournal of Immunology
Volume135
Issue number4
StatePublished - 1985
Externally publishedYes

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Malaria
Epitopes
Parasites
Monoclonal Antibodies
Immunoradiometric Assay
Plasmodium vivax
Sporozoites
Falciparum Malaria
Polyacrylamide Gel Electrophoresis
Proteins
Western Blotting

ASJC Scopus subject areas

  • Immunology

Cite this

Zavala, F. P., Masuda, A., Graves, P. M., Nussenzweig, V., & Nussenzweig, R. S. (1985). Ubiquity of the repetitive epitope of the CS protein in different isolates of human malaria parasites. Journal of Immunology, 135(4), 2790-2793.

Ubiquity of the repetitive epitope of the CS protein in different isolates of human malaria parasites. / Zavala, Fidel P; Masuda, A.; Graves, P. M.; Nussenzweig, V.; Nussenzweig, R. S.

In: Journal of Immunology, Vol. 135, No. 4, 1985, p. 2790-2793.

Research output: Contribution to journalArticle

Zavala, FP, Masuda, A, Graves, PM, Nussenzweig, V & Nussenzweig, RS 1985, 'Ubiquity of the repetitive epitope of the CS protein in different isolates of human malaria parasites', Journal of Immunology, vol. 135, no. 4, pp. 2790-2793.
Zavala, Fidel P ; Masuda, A. ; Graves, P. M. ; Nussenzweig, V. ; Nussenzweig, R. S. / Ubiquity of the repetitive epitope of the CS protein in different isolates of human malaria parasites. In: Journal of Immunology. 1985 ; Vol. 135, No. 4. pp. 2790-2793.
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