Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans

J. Seriwatana, P. Echeverria, D. N. Taylor, L. Rasrinaul, J. E. Brown, J. S M Peiris, C. L. Clayton

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Abstract

Heat-labile enterotoxin (LT)-producing Escherichia coli strains, as identified by the Y1 adrenal cell assay, were examined with a DNA probe coding for type I and type II LTs. Of 236 LT-producing E. coli isolates, 60% hybridized with LT-I, 17% hybridized with LT-II, and 23% did not hybridize with either probe and no longer produced LT as determined by the Y1 adrenal cell assay. These isolates presumably lost plasmids coding for LT-I during storage. A total of 75% of LT-producing E. coli isolates (27 of 36) from cows, 64% of LT-producing E. coli isolates (7 of 11) from buffalo, 31% of LT-producing E. coli isolates (4 of 13) from beef obtained in markets, and 2% of LT-producing E. coli isolates (3 of 168) from humans contained genes coding for LT-II. Genes coding for LT-II were not found in 50 LT-I-producing and heat-stable enterotoxin-producing E. coli isolates from 11 children with diarrhea and 44 LT-nonproducing and heat-stable enterotoxin-producing E. coli isolates from 12 other children with diarrhea. A total of 9% of LT-II-producing E. coli isolates (3 of 34) from cows and buffalo hybridized with DNA probes for genes coding for verocytotoxin 2 (VT2), and 18% (6 of 34) hybridized with a DNA probe coding for enterohemorrhagic E. coli (EHEC) adhesin fimbriae. E. coli SA-53, the original isolate in which LT-II was found contained genes coding for VT2 and EHEC adhesin fimbriae. Five VT-producing, LT-II-producing E. coli isolates that hybridized with the EHEC probe did not contain DNA sequences coding for VT1 or VT2. LT-II-producing E. coli strains were frequently isolated from cattle and buffalo but were rarely isolated from humans.

Original languageEnglish (US)
Pages (from-to)1158-1161
Number of pages4
JournalInfection and Immunity
Volume56
Issue number5
Publication statusPublished - 1988
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

Cite this

Seriwatana, J., Echeverria, P., Taylor, D. N., Rasrinaul, L., Brown, J. E., Peiris, J. S. M., & Clayton, C. L. (1988). Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans. Infection and Immunity, 56(5), 1158-1161.