Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans

J. Seriwatana, P. Echeverria, D. N. Taylor, L. Rasrinaul, J. E. Brown, J. S M Peiris, C. L. Clayton

Research output: Contribution to journalArticle

Abstract

Heat-labile enterotoxin (LT)-producing Escherichia coli strains, as identified by the Y1 adrenal cell assay, were examined with a DNA probe coding for type I and type II LTs. Of 236 LT-producing E. coli isolates, 60% hybridized with LT-I, 17% hybridized with LT-II, and 23% did not hybridize with either probe and no longer produced LT as determined by the Y1 adrenal cell assay. These isolates presumably lost plasmids coding for LT-I during storage. A total of 75% of LT-producing E. coli isolates (27 of 36) from cows, 64% of LT-producing E. coli isolates (7 of 11) from buffalo, 31% of LT-producing E. coli isolates (4 of 13) from beef obtained in markets, and 2% of LT-producing E. coli isolates (3 of 168) from humans contained genes coding for LT-II. Genes coding for LT-II were not found in 50 LT-I-producing and heat-stable enterotoxin-producing E. coli isolates from 11 children with diarrhea and 44 LT-nonproducing and heat-stable enterotoxin-producing E. coli isolates from 12 other children with diarrhea. A total of 9% of LT-II-producing E. coli isolates (3 of 34) from cows and buffalo hybridized with DNA probes for genes coding for verocytotoxin 2 (VT2), and 18% (6 of 34) hybridized with a DNA probe coding for enterohemorrhagic E. coli (EHEC) adhesin fimbriae. E. coli SA-53, the original isolate in which LT-II was found contained genes coding for VT2 and EHEC adhesin fimbriae. Five VT-producing, LT-II-producing E. coli isolates that hybridized with the EHEC probe did not contain DNA sequences coding for VT1 or VT2. LT-II-producing E. coli strains were frequently isolated from cattle and buffalo but were rarely isolated from humans.

Original languageEnglish (US)
Pages (from-to)1158-1161
Number of pages4
JournalInfection and Immunity
Volume56
Issue number5
StatePublished - 1988
Externally publishedYes

Fingerprint

Enterotoxins
Hot Temperature
Escherichia coli
Enterohemorrhagic Escherichia coli
Buffaloes
Escherichia coli Adhesins
DNA Probes
Diarrhea
Genes
Plasmids

ASJC Scopus subject areas

  • Immunology

Cite this

Seriwatana, J., Echeverria, P., Taylor, D. N., Rasrinaul, L., Brown, J. E., Peiris, J. S. M., & Clayton, C. L. (1988). Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans. Infection and Immunity, 56(5), 1158-1161.

Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans. / Seriwatana, J.; Echeverria, P.; Taylor, D. N.; Rasrinaul, L.; Brown, J. E.; Peiris, J. S M; Clayton, C. L.

In: Infection and Immunity, Vol. 56, No. 5, 1988, p. 1158-1161.

Research output: Contribution to journalArticle

Seriwatana, J, Echeverria, P, Taylor, DN, Rasrinaul, L, Brown, JE, Peiris, JSM & Clayton, CL 1988, 'Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans', Infection and Immunity, vol. 56, no. 5, pp. 1158-1161.
Seriwatana J, Echeverria P, Taylor DN, Rasrinaul L, Brown JE, Peiris JSM et al. Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans. Infection and Immunity. 1988;56(5):1158-1161.
Seriwatana, J. ; Echeverria, P. ; Taylor, D. N. ; Rasrinaul, L. ; Brown, J. E. ; Peiris, J. S M ; Clayton, C. L. / Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans. In: Infection and Immunity. 1988 ; Vol. 56, No. 5. pp. 1158-1161.
@article{318140bda641464bb4187d6c3c53829e,
title = "Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans",
abstract = "Heat-labile enterotoxin (LT)-producing Escherichia coli strains, as identified by the Y1 adrenal cell assay, were examined with a DNA probe coding for type I and type II LTs. Of 236 LT-producing E. coli isolates, 60{\%} hybridized with LT-I, 17{\%} hybridized with LT-II, and 23{\%} did not hybridize with either probe and no longer produced LT as determined by the Y1 adrenal cell assay. These isolates presumably lost plasmids coding for LT-I during storage. A total of 75{\%} of LT-producing E. coli isolates (27 of 36) from cows, 64{\%} of LT-producing E. coli isolates (7 of 11) from buffalo, 31{\%} of LT-producing E. coli isolates (4 of 13) from beef obtained in markets, and 2{\%} of LT-producing E. coli isolates (3 of 168) from humans contained genes coding for LT-II. Genes coding for LT-II were not found in 50 LT-I-producing and heat-stable enterotoxin-producing E. coli isolates from 11 children with diarrhea and 44 LT-nonproducing and heat-stable enterotoxin-producing E. coli isolates from 12 other children with diarrhea. A total of 9{\%} of LT-II-producing E. coli isolates (3 of 34) from cows and buffalo hybridized with DNA probes for genes coding for verocytotoxin 2 (VT2), and 18{\%} (6 of 34) hybridized with a DNA probe coding for enterohemorrhagic E. coli (EHEC) adhesin fimbriae. E. coli SA-53, the original isolate in which LT-II was found contained genes coding for VT2 and EHEC adhesin fimbriae. Five VT-producing, LT-II-producing E. coli isolates that hybridized with the EHEC probe did not contain DNA sequences coding for VT1 or VT2. LT-II-producing E. coli strains were frequently isolated from cattle and buffalo but were rarely isolated from humans.",
author = "J. Seriwatana and P. Echeverria and Taylor, {D. N.} and L. Rasrinaul and Brown, {J. E.} and Peiris, {J. S M} and Clayton, {C. L.}",
year = "1988",
language = "English (US)",
volume = "56",
pages = "1158--1161",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans

AU - Seriwatana, J.

AU - Echeverria, P.

AU - Taylor, D. N.

AU - Rasrinaul, L.

AU - Brown, J. E.

AU - Peiris, J. S M

AU - Clayton, C. L.

PY - 1988

Y1 - 1988

N2 - Heat-labile enterotoxin (LT)-producing Escherichia coli strains, as identified by the Y1 adrenal cell assay, were examined with a DNA probe coding for type I and type II LTs. Of 236 LT-producing E. coli isolates, 60% hybridized with LT-I, 17% hybridized with LT-II, and 23% did not hybridize with either probe and no longer produced LT as determined by the Y1 adrenal cell assay. These isolates presumably lost plasmids coding for LT-I during storage. A total of 75% of LT-producing E. coli isolates (27 of 36) from cows, 64% of LT-producing E. coli isolates (7 of 11) from buffalo, 31% of LT-producing E. coli isolates (4 of 13) from beef obtained in markets, and 2% of LT-producing E. coli isolates (3 of 168) from humans contained genes coding for LT-II. Genes coding for LT-II were not found in 50 LT-I-producing and heat-stable enterotoxin-producing E. coli isolates from 11 children with diarrhea and 44 LT-nonproducing and heat-stable enterotoxin-producing E. coli isolates from 12 other children with diarrhea. A total of 9% of LT-II-producing E. coli isolates (3 of 34) from cows and buffalo hybridized with DNA probes for genes coding for verocytotoxin 2 (VT2), and 18% (6 of 34) hybridized with a DNA probe coding for enterohemorrhagic E. coli (EHEC) adhesin fimbriae. E. coli SA-53, the original isolate in which LT-II was found contained genes coding for VT2 and EHEC adhesin fimbriae. Five VT-producing, LT-II-producing E. coli isolates that hybridized with the EHEC probe did not contain DNA sequences coding for VT1 or VT2. LT-II-producing E. coli strains were frequently isolated from cattle and buffalo but were rarely isolated from humans.

AB - Heat-labile enterotoxin (LT)-producing Escherichia coli strains, as identified by the Y1 adrenal cell assay, were examined with a DNA probe coding for type I and type II LTs. Of 236 LT-producing E. coli isolates, 60% hybridized with LT-I, 17% hybridized with LT-II, and 23% did not hybridize with either probe and no longer produced LT as determined by the Y1 adrenal cell assay. These isolates presumably lost plasmids coding for LT-I during storage. A total of 75% of LT-producing E. coli isolates (27 of 36) from cows, 64% of LT-producing E. coli isolates (7 of 11) from buffalo, 31% of LT-producing E. coli isolates (4 of 13) from beef obtained in markets, and 2% of LT-producing E. coli isolates (3 of 168) from humans contained genes coding for LT-II. Genes coding for LT-II were not found in 50 LT-I-producing and heat-stable enterotoxin-producing E. coli isolates from 11 children with diarrhea and 44 LT-nonproducing and heat-stable enterotoxin-producing E. coli isolates from 12 other children with diarrhea. A total of 9% of LT-II-producing E. coli isolates (3 of 34) from cows and buffalo hybridized with DNA probes for genes coding for verocytotoxin 2 (VT2), and 18% (6 of 34) hybridized with a DNA probe coding for enterohemorrhagic E. coli (EHEC) adhesin fimbriae. E. coli SA-53, the original isolate in which LT-II was found contained genes coding for VT2 and EHEC adhesin fimbriae. Five VT-producing, LT-II-producing E. coli isolates that hybridized with the EHEC probe did not contain DNA sequences coding for VT1 or VT2. LT-II-producing E. coli strains were frequently isolated from cattle and buffalo but were rarely isolated from humans.

UR - http://www.scopus.com/inward/record.url?scp=0023929117&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023929117&partnerID=8YFLogxK

M3 - Article

C2 - 2833443

AN - SCOPUS:0023929117

VL - 56

SP - 1158

EP - 1161

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 5

ER -