Type II corticosteroid receptor-like immunoreactivity in the rat cerebellar cortex: Differential regulation by corticosterone

Rexford S. Ahima, Clifford N.B. Tagoe, Richard E. Harlana

Research output: Contribution to journalArticle

Abstract

Type II corticosteroid receptor-like immunoreactivity (type Il-ir) was localized at the light- and electron-microscopic levels in the rat cerebellar cortex using BUGR2 monoclonal antibody. In intact rats, type Il-ir was observed in the nuclei of basket, stellate, Golgi and Purkinje cells. After 1 week of adrenalectomy, type Il-ir was barely resolvable in basket, stellate, Golgi and most Purkinje cells. Vermal Purkinje cells showed intense nuclear and cytoplasmic type Il-ir. After 4 weeks of adrenalectomy, type Il-ir was markedly reduced in most vermal Purkinje cells, however a few in lobules 1-3 and 9 and 10 retained diffuse immunoreactivity. Acute treatment with corticosterone restored nuclear type Il-ir to basket, stellate, Golgi and Purkinje cells in the cerebellar hemispheres, flocculi and paraflocculi. In the vermis most Purkinje cells showed nuclear type Il-ir following corticosterone treatment. Diffuse type Il- ir was, however, retained in a few Purkinje cells in vermal lobules 1-3, 9 and 10. The distribution of GABA-like immunoreactivity (GABA-ir) in the cerebellar cortex was not altered by adrenalectomy or acute corticosterone treatment. 30-60% of type Il-ir Purkinje cells in intact rats were GABA-ir. In adre- nalectomized and corticosterone-treated rats, a similar proportion of type Il-ir Purkinje cells were GABA-ir. The differential regulation of neuronal type Il-ir in the cerebellum by corticosterone may account for some of the known effects of glucocorticoids on motor coordination.

Original languageEnglish (US)
Pages (from-to)683-694
Number of pages12
JournalNeuroendocrinology
Volume55
Issue number6
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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Keywords

  • Glucocorticoids
  • Golgi cells
  • Purkinje cells

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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