Type 2 rice metallothionein-like gene has two introns

Wen Ming Che, Hsing Mei Hsieh, P. C. Huang

Research output: Contribution to journalArticlepeer-review

Abstract

A type 2 rice metallothionein-like gene was isolated from root by PCR and sequenced. The PCR fragment was designated as pcr1460, which overlaps with OsMT-2, a cDNA sequence previously characterized, with the presence of two additional segments, 583 and 613 bp in length. These segments are recognized as introns which divide the coding sequence into three exons, 65, 78 and 106 base pairs in length. The sequences flanking the introns conform with the GT/AG rule for splice junctions, and one exonic open reading frame can be identified in each of the introns. The observation that MT-like gene has two introns is the first of such a finding obtained from monocotyle-donous plants.

Original languageEnglish (US)
Pages (from-to)223-228
Number of pages6
JournalMitochondrial DNA
Volume8
Issue number4
DOIs
StatePublished - 1998

Keywords

  • Exonic ORF
  • intron
  • metallothionein

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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