Type 2 diabetes dysregulates glucose metabolism in cardiac progenitor cells

Joshua K. Salabei, Pawel K. Lorkiewicz, Parul Mehra, Andrew A. Gibb, Petra Haberzettl, Kyung U. Hong, Xiaoli Wei, Xiang Zhang, Qianhong Li, Marcin Wysoczynski, Roberto Bolli, Aruni Bhatnagar, Bradford G. Hill

Research output: Contribution to journalArticle

Abstract

Type 2 diabetes is associated with increased mortality and progression to heart failure. Recent studies suggest that diabetes also impairs reparative responses after cell therapy. In this study, we examined potential mechanisms by which diabetes affects cardiac progenitor cells (CPCs). CPCs isolated from the diabetic heart showed diminished proliferation, a propensity for cell death, and a pro-adipogenic phenotype. The diabetic CPCs were insulin-resistant, and they showed higher energetic reliance on glycolysis, which was associated with up-regulation of the pro-glycolytic enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3). In WT CPCs, expression of a mutant form of PFKFB, which mimics PFKFB3 activity and increases glycolytic rate, was sufficient to phenocopy the mitochondrial and proliferative deficiencies found in diabetic cells. Consistent with activation of phosphofructokinase in diabetic cells, stable isotope carbon tracing in diabetic CPCs showed dysregulation of the pentose phosphate and glycero(phospho)lipid synthesis pathways. We describe diabetes-induced dysregulation of carbon partitioning using stable isotope metabolomics-based coupling quotients, which relate relative flux values between metabolic pathways. These findings suggest that diabetes causes an imbalance in glucose carbon allocation by uncoupling biosynthetic pathway activity, which could diminish the efficacy of CPCs for myocardial repair.

Original languageEnglish (US)
Pages (from-to)13634-13648
Number of pages15
JournalJournal of Biological Chemistry
Volume291
Issue number26
DOIs
StatePublished - Jun 24 2016
Externally publishedYes

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Medical problems
Metabolism
Type 2 Diabetes Mellitus
Stem Cells
Glucose
Phosphofructokinase-2
Carbon
Carbon Isotopes
Pentoses
Phosphofructokinases
Cell death
Metabolomics
Isotopes
Biosynthetic Pathways
Glycolysis
Cell- and Tissue-Based Therapy
Metabolic Networks and Pathways
Repair
Phosphotransferases
Chemical activation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Salabei, J. K., Lorkiewicz, P. K., Mehra, P., Gibb, A. A., Haberzettl, P., Hong, K. U., ... Hill, B. G. (2016). Type 2 diabetes dysregulates glucose metabolism in cardiac progenitor cells. Journal of Biological Chemistry, 291(26), 13634-13648. https://doi.org/10.1074/jbc.M116.722496

Type 2 diabetes dysregulates glucose metabolism in cardiac progenitor cells. / Salabei, Joshua K.; Lorkiewicz, Pawel K.; Mehra, Parul; Gibb, Andrew A.; Haberzettl, Petra; Hong, Kyung U.; Wei, Xiaoli; Zhang, Xiang; Li, Qianhong; Wysoczynski, Marcin; Bolli, Roberto; Bhatnagar, Aruni; Hill, Bradford G.

In: Journal of Biological Chemistry, Vol. 291, No. 26, 24.06.2016, p. 13634-13648.

Research output: Contribution to journalArticle

Salabei, JK, Lorkiewicz, PK, Mehra, P, Gibb, AA, Haberzettl, P, Hong, KU, Wei, X, Zhang, X, Li, Q, Wysoczynski, M, Bolli, R, Bhatnagar, A & Hill, BG 2016, 'Type 2 diabetes dysregulates glucose metabolism in cardiac progenitor cells', Journal of Biological Chemistry, vol. 291, no. 26, pp. 13634-13648. https://doi.org/10.1074/jbc.M116.722496
Salabei JK, Lorkiewicz PK, Mehra P, Gibb AA, Haberzettl P, Hong KU et al. Type 2 diabetes dysregulates glucose metabolism in cardiac progenitor cells. Journal of Biological Chemistry. 2016 Jun 24;291(26):13634-13648. https://doi.org/10.1074/jbc.M116.722496
Salabei, Joshua K. ; Lorkiewicz, Pawel K. ; Mehra, Parul ; Gibb, Andrew A. ; Haberzettl, Petra ; Hong, Kyung U. ; Wei, Xiaoli ; Zhang, Xiang ; Li, Qianhong ; Wysoczynski, Marcin ; Bolli, Roberto ; Bhatnagar, Aruni ; Hill, Bradford G. / Type 2 diabetes dysregulates glucose metabolism in cardiac progenitor cells. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 26. pp. 13634-13648.
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