Two-stage elevation of cell-free fetal DNA in maternal sera before onset of preeclampsia

Richard J. Levine; Cong Qian; Erik S. Leshane; Kai F. Yu; Lucinda J. England; Enrique F. Schisterman; Tuangsit Wataganara; Roberto Romero; Diana W. Bianchi

doi:10.1016/j.ajog.2003.12.019

Two-stage elevation of cell-free fetal DNA in maternal sera before onset of preeclampsia

Richard J. Levine, Cong Qian, Erik S. Leshane, Kai F. Yu, Lucinda J. England, Enrique F. Schisterman, Tuangsit Wataganara, Roberto Romero, Diana W. Bianchi

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: The purpose was to determine whether preeclampsia (PE) is caused by microfragments of syncytial trophoblast shed into the maternal circulation that stimulate an exaggerated inflammatory response. Study design: A nested case control study was performed within the Calcium for Preeclampsia Prevention trial cohort of healthy nulliparous women. Each preeclampsia case was matched to 1 normotensive control. One hundred twenty pairs were randomly chosen for analysis of serum cell-free fetal DNA (cffDNA), a marker of placental debris, and C-reactive protein (CRP), a marker of inflammation, in all 658 specimens obtained before labor. Results: At 29 to 41 weeks of gestation, cffDNA concentrations were significantly higher after preeclampsia than before (219 vs 112 genome equivalents [GE]/mL, P

Original language	English (US)
Pages (from-to)	707-713
Number of pages	7
Journal	American Journal of Obstetrics and Gynecology
Volume	190
Issue number	3
DOIs	https://doi.org/10.1016/j.ajog.2003.12.019
State	Published - Mar 2004
Externally published	Yes

Keywords

C-reactive protein
Cell-free fetal DNA
Inflammation
Placenta
Preeclampsia

ASJC Scopus subject areas

Medicine(all)
Obstetrics and Gynecology

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10.1016/j.ajog.2003.12.019

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title = "Two-stage elevation of cell-free fetal DNA in maternal sera before onset of preeclampsia",

abstract = "Objective: The purpose was to determine whether preeclampsia (PE) is caused by microfragments of syncytial trophoblast shed into the maternal circulation that stimulate an exaggerated inflammatory response. Study design: A nested case control study was performed within the Calcium for Preeclampsia Prevention trial cohort of healthy nulliparous women. Each preeclampsia case was matched to 1 normotensive control. One hundred twenty pairs were randomly chosen for analysis of serum cell-free fetal DNA (cffDNA), a marker of placental debris, and C-reactive protein (CRP), a marker of inflammation, in all 658 specimens obtained before labor. Results: At 29 to 41 weeks of gestation, cffDNA concentrations were significantly higher after preeclampsia than before (219 vs 112 genome equivalents [GE]/mL, P",

keywords = "C-reactive protein, Cell-free fetal DNA, Inflammation, Placenta, Preeclampsia",

author = "Levine, {Richard J.} and Cong Qian and Leshane, {Erik S.} and Yu, {Kai F.} and England, {Lucinda J.} and Schisterman, {Enrique F.} and Tuangsit Wataganara and Roberto Romero and Bianchi, {Diana W.}",

year = "2004",

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doi = "10.1016/j.ajog.2003.12.019",

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pages = "707--713",

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AU - Levine, Richard J.

AU - Qian, Cong

AU - Leshane, Erik S.

AU - Yu, Kai F.

AU - England, Lucinda J.

AU - Schisterman, Enrique F.

AU - Wataganara, Tuangsit

AU - Romero, Roberto

AU - Bianchi, Diana W.

PY - 2004/3

Y1 - 2004/3

N2 - Objective: The purpose was to determine whether preeclampsia (PE) is caused by microfragments of syncytial trophoblast shed into the maternal circulation that stimulate an exaggerated inflammatory response. Study design: A nested case control study was performed within the Calcium for Preeclampsia Prevention trial cohort of healthy nulliparous women. Each preeclampsia case was matched to 1 normotensive control. One hundred twenty pairs were randomly chosen for analysis of serum cell-free fetal DNA (cffDNA), a marker of placental debris, and C-reactive protein (CRP), a marker of inflammation, in all 658 specimens obtained before labor. Results: At 29 to 41 weeks of gestation, cffDNA concentrations were significantly higher after preeclampsia than before (219 vs 112 genome equivalents [GE]/mL, P

AB - Objective: The purpose was to determine whether preeclampsia (PE) is caused by microfragments of syncytial trophoblast shed into the maternal circulation that stimulate an exaggerated inflammatory response. Study design: A nested case control study was performed within the Calcium for Preeclampsia Prevention trial cohort of healthy nulliparous women. Each preeclampsia case was matched to 1 normotensive control. One hundred twenty pairs were randomly chosen for analysis of serum cell-free fetal DNA (cffDNA), a marker of placental debris, and C-reactive protein (CRP), a marker of inflammation, in all 658 specimens obtained before labor. Results: At 29 to 41 weeks of gestation, cffDNA concentrations were significantly higher after preeclampsia than before (219 vs 112 genome equivalents [GE]/mL, P

KW - C-reactive protein

KW - Cell-free fetal DNA

KW - Inflammation

KW - Placenta

KW - Preeclampsia

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Two-stage elevation of cell-free fetal DNA in maternal sera before onset of preeclampsia

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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