Two-site ELISA for the quantitative determination of fatty acid synthase

Young Wang, Francis P. Kuhajda, Lori J. Sokoll, Daniel W. Chan

Research output: Contribution to journalArticle

Abstract

Fatty acid synthase (FAS) is an enzyme which plays a central role in the de novo biosynthesis of fatty acids. FAS is selectively expressed in certain human cancers and therefore is a putative tumor marker. We developed an enzyme-linked immunosorbent assay (ELISA) for measuring FAS, and investigated its expression and clinical features. In this two-site sandwich ELISA, a polyclonal antibody was used as a capture on Nunc MaxiSorp ELISA/EIA modules and a monoclonal antibody labeled with biotin was used as a signal antibody. The assay was linear with no cross-reactivity with other tumor markers. The within- and between-run CVs were <10%, and the detection limit was 0.15 arbitrary Units/l. Recoveries were 92.4-105.1%. FAS was stable in buffer at 4°C for more than 10 days and stable at 37°C for 2 days. In human serum, FAS levels were significantly higher in patients with breast (1.01±0.71 Units/l, mean±S.D.), prostate (0.79±0.76 Units/l), colon (0.89±0.49 Units/l), and ovarian (0.84±0.9 Units/l) cancers compared to normal subjects (0.27±0.09 Units/l, P<0.01). This assay is sensitive, accurate, and precise and can distinguish between patients with various types of cancer and normal subjects.

Original languageEnglish (US)
Pages (from-to)107-115
Number of pages9
JournalClinica Chimica Acta
Volume304
Issue number1-2
DOIs
StatePublished - Feb 19 2001

Keywords

  • Enzyme
  • Fatty acid synthase
  • Immunoassay
  • Tumor marker

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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