@article{5503ebf2d9c544b28b3b24f91d0dee7d,
title = "Two Rab2 interactors regulate dense-core vesicle maturation",
abstract = "Peptide neuromodulators are released from a unique organelle: the dense-core vesicle. Dense-core vesicles are generated at the trans-Golgi and then sort cargo during maturation before being secreted. To identify proteins that act in this pathway, we performed a genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We identified two conserved Rab2-binding proteins: RUND-1, a RUN domain protein, and CCCP-1, a coiled-coil protein. RUND-1 and CCCP-1 colocalize with RAB-2 at the Golgi, and rab-2, rund-1, and cccp-1 mutants have similar defects in sorting soluble and transmembrane dense-core vesicle cargos. RUND-1 also interacts with the Rab2 GAP protein TBC-8 and the BAR domain protein RIC-19, a RAB-2 effector. In summary, a pathway of conserved proteins controls the maturation of dense-core vesicles at the trans-Golgi network.",
author = "Michael Ailion and Mandy Hannemann and Susan Dalton and Andrea Pappas and Shigeki Watanabe and Jan Hegermann and Qiang Liu and Han, {Hsiao Fen} and Mingyu Gu and Goulding, {Morgan Q.} and Nikhil Sasidharan and Kim Schuske and Patrick Hullett and Stefan Eimer and Jorgensen, {Erik M.}",
note = "Funding Information: We thank K. Iwasaki, K. Miller, J. Kaplan, Z. Zhou, S. Mitani, B. Grant, C. Fr{\o}kj{\ae}r-Jensen, G. Hollopeter, and P. McEachern for strains and plasmids; Y. Kohara for cDNA clones; the Sanger Center for cosmids; R. Rawson for help with confocal microscopy; D. Hobson for data analysis algorithms; and I. Topalidou for comments on the manuscript. Strains were provided by the CGC (P40 OD010440). M.A. was supported by Helen Hay Whitney and by NIH R00MH082109. M.H. was supported by the University of G{\"o}ttingen GGNB program (DFG grant GSC 226/1). S.E. was supported by German Research Foundation CMPB and the European Neuroscience Institute. This work was supported by NIH NS034307 and NSF IOS-0920069 to E.M.J. ",
year = "2014",
month = apr,
day = "2",
doi = "10.1016/j.neuron.2014.02.017",
language = "English (US)",
volume = "82",
pages = "167--180",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "1",
}