Two-photon fluorescence imaging of embryo with much less damage than confocal imaging

Bian Liu, Hui Xu, Lei Jin, Hui Ma, Die Yan Chen

Research output: Contribution to journalConference article

Abstract

Two-photon Laser Scanning Microscopy (TPLSM) is a novel technique based on the two-photon excitation of fluorophore. In this paper, TPLSM and traditional confocal microscopy are introduced. And the influence of femtosecond near-infrared (NIR) illumination on mouse embryos is investigated for the first time. The result shows that NIR laser has much less damage to embryos than blue laser and proves that TPLSM is superior to conventional confocal microscopy in keeping sample alive. TPLSM enables us to make a continuous observation for a longer time on embryogenesis.

Original languageEnglish (US)
Pages (from-to)126-129
Number of pages4
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4919
DOIs
StatePublished - Jan 1 2002
Externally publishedYes
EventAdvanced Materials and Devices for Sensing and Imaging - Shanghai, China
Duration: Oct 17 2002Oct 18 2002

Fingerprint

Confocal
embryos
Embryo
Laser Scanning
Fluorescence
Photon
Photons
Damage
Microscopy
Imaging
damage
microscopy
Microscopic examination
Imaging techniques
fluorescence
Lasers
photons
Scanning
Confocal Microscopy
Confocal microscopy

Keywords

  • Confocal
  • Damage
  • Embryo
  • Microscopy
  • NIR
  • TPLSM
  • Two-photon

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Two-photon fluorescence imaging of embryo with much less damage than confocal imaging. / Liu, Bian; Xu, Hui; Jin, Lei; Ma, Hui; Chen, Die Yan.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 4919, 01.01.2002, p. 126-129.

Research output: Contribution to journalConference article

@article{d7a4e0f631c9463392409719d530e4af,
title = "Two-photon fluorescence imaging of embryo with much less damage than confocal imaging",
abstract = "Two-photon Laser Scanning Microscopy (TPLSM) is a novel technique based on the two-photon excitation of fluorophore. In this paper, TPLSM and traditional confocal microscopy are introduced. And the influence of femtosecond near-infrared (NIR) illumination on mouse embryos is investigated for the first time. The result shows that NIR laser has much less damage to embryos than blue laser and proves that TPLSM is superior to conventional confocal microscopy in keeping sample alive. TPLSM enables us to make a continuous observation for a longer time on embryogenesis.",
keywords = "Confocal, Damage, Embryo, Microscopy, NIR, TPLSM, Two-photon",
author = "Bian Liu and Hui Xu and Lei Jin and Hui Ma and Chen, {Die Yan}",
year = "2002",
month = "1",
day = "1",
doi = "10.1117/12.465727",
language = "English (US)",
volume = "4919",
pages = "126--129",
journal = "Proceedings of SPIE - The International Society for Optical Engineering",
issn = "0277-786X",
publisher = "SPIE",

}

TY - JOUR

T1 - Two-photon fluorescence imaging of embryo with much less damage than confocal imaging

AU - Liu, Bian

AU - Xu, Hui

AU - Jin, Lei

AU - Ma, Hui

AU - Chen, Die Yan

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Two-photon Laser Scanning Microscopy (TPLSM) is a novel technique based on the two-photon excitation of fluorophore. In this paper, TPLSM and traditional confocal microscopy are introduced. And the influence of femtosecond near-infrared (NIR) illumination on mouse embryos is investigated for the first time. The result shows that NIR laser has much less damage to embryos than blue laser and proves that TPLSM is superior to conventional confocal microscopy in keeping sample alive. TPLSM enables us to make a continuous observation for a longer time on embryogenesis.

AB - Two-photon Laser Scanning Microscopy (TPLSM) is a novel technique based on the two-photon excitation of fluorophore. In this paper, TPLSM and traditional confocal microscopy are introduced. And the influence of femtosecond near-infrared (NIR) illumination on mouse embryos is investigated for the first time. The result shows that NIR laser has much less damage to embryos than blue laser and proves that TPLSM is superior to conventional confocal microscopy in keeping sample alive. TPLSM enables us to make a continuous observation for a longer time on embryogenesis.

KW - Confocal

KW - Damage

KW - Embryo

KW - Microscopy

KW - NIR

KW - TPLSM

KW - Two-photon

UR - http://www.scopus.com/inward/record.url?scp=0036442060&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036442060&partnerID=8YFLogxK

U2 - 10.1117/12.465727

DO - 10.1117/12.465727

M3 - Conference article

AN - SCOPUS:0036442060

VL - 4919

SP - 126

EP - 129

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

SN - 0277-786X

ER -