Two-photon fluorescence imaging of embryo with much less damage than confocal imaging

Bian Liu, Hui Xu, Lei Jin, Hui Ma, Die Yan Chen

Research output: Contribution to journalConference article

Abstract

Two-photon Laser Scanning Microscopy (TPLSM) is a novel technique based on the two-photon excitation of fluorophore. In this paper, TPLSM and traditional confocal microscopy are introduced. And the influence of femtosecond near-infrared (NIR) illumination on mouse embryos is investigated for the first time. The result shows that NIR laser has much less damage to embryos than blue laser and proves that TPLSM is superior to conventional confocal microscopy in keeping sample alive. TPLSM enables us to make a continuous observation for a longer time on embryogenesis.

Original languageEnglish (US)
Pages (from-to)126-129
Number of pages4
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4919
DOIs
StatePublished - 2002
EventAdvanced Materials and Devices for Sensing and Imaging - Shanghai, China
Duration: Oct 17 2002Oct 18 2002

Keywords

  • Confocal
  • Damage
  • Embryo
  • Microscopy
  • NIR
  • TPLSM
  • Two-photon

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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