Two-photon excitation of quantum-dot-based fluorescence resonance energy transfer and its applications

Aaron R. Clapp, Thomas Pons, Igor L. Medintz, James B. Delehanty, Joseph S. Melinger, Theresa Tiefenbrunn, Philip E. Dawson, Brent R. Fisher, Brian O'Rourke, Hedi Mattoussi

Research output: Contribution to journalArticlepeer-review


The efficient resonance energy transfer between luminescent Quantum Dots (QD) and proximal dye acceptors driven by a two-photon process using sub-band excitation energy was demonstrated. The fluorescence resonance energy transfer (FRET) process between QDs and proximal dyes exploits very high two-photon action cross section of QDs compared to those of conventional dyes and provides signal-to-background ratios in FRET imaging of cells and tissue samples. Two-photon action cross-sectional values measured for QDs in water solution shows that the measured values for QDs are 2-3 orders of magnitude higher than Cyanine. The loss of QD emission is specifically due to self-assembly of the dye-labeled protein on the nanocrystal surface,which positions the dye acceptors close to the QD donor. QDs conjugated with cell-penetrating-peptides (CPP) and Cy3-labeleld peptide and assembled at average ratios shows an efficient FRET from QDs to Cy3.

Original languageEnglish (US)
Pages (from-to)1921-1926
Number of pages6
JournalAdvanced Materials
Issue number15
StatePublished - Aug 3 2007

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering


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