Two mechanisms of antigen-specific apoptosis of myelin basic protein (MBP)-specific T lymphocytes derived from multiple sclerosis patients and normal individuals

C. M. Pelfrey, L. R. Tranquill, S. A. Boehme, H. F. McFarland, M. J. Lenardo

Research output: Contribution to journalArticle

Abstract

Several stimuli induce mature T cells to undergo apoptosis or programmed cell death (PCD) including specific Ag. We have demonstrated previously that Ag induces the death of encephalitogenic T cells in vitro and deletion in vivo, leading to amelioration of autoimmune encephalomyelitis. We have now examined whether activated, myelin basic protein (MBP)-specific human T cells may be eliminated by Ag-induced PCD. We demonstrate that activated MBP- specific T cell lines (TCL) undergo the classic nuclear morphologic changes and DNA fragmentation characteristic of apoptosis when given a TCR challenge. We found evidence that two mechanisms led to apoptosis: a propriocidal mechanism that was highly Ag-specific and dependent on the dose of exogenously added rIL-2, and a cytolytic mechanism in which MBP-specific TCL lysed B cell targets and engaged in considerable 'fratricidal' cytolysis of other MBP-specific T cells. These two pathways leading to MBP-specific apoptotic death could be distinguished by their glucocorticoid sensitivity. Glucocorticoid treatment significantly blocked MBP-induced propriocidal apoptosis but had no effect on T cell cytolysis of B cell targets. Although it has been proposed that autoimmune disease could result from the failure of normal deletional mechanisms, this preliminary survey of MBP-reactive mature TCL from multiple sclerosis patients revealed that such cells are highly susceptible to TCR-induced PCD and comparable with TCL from normal subjects. Thus, therapeutic strategies based on Ag-induced PCD of T lymphocytes may be feasible in man.

Original languageEnglish (US)
Pages (from-to)6191-6202
Number of pages12
JournalJournal of Immunology
Volume154
Issue number11
StatePublished - 1995
Externally publishedYes

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Myelin Basic Protein
Multiple Sclerosis
Apoptosis
T-Lymphocytes
Antigens
Cell Death
Cell Line
Glucocorticoids
B-Lymphocytes
Encephalomyelitis
DNA Fragmentation
Autoimmune Diseases

ASJC Scopus subject areas

  • Immunology

Cite this

Two mechanisms of antigen-specific apoptosis of myelin basic protein (MBP)-specific T lymphocytes derived from multiple sclerosis patients and normal individuals. / Pelfrey, C. M.; Tranquill, L. R.; Boehme, S. A.; McFarland, H. F.; Lenardo, M. J.

In: Journal of Immunology, Vol. 154, No. 11, 1995, p. 6191-6202.

Research output: Contribution to journalArticle

Pelfrey, C. M. ; Tranquill, L. R. ; Boehme, S. A. ; McFarland, H. F. ; Lenardo, M. J. / Two mechanisms of antigen-specific apoptosis of myelin basic protein (MBP)-specific T lymphocytes derived from multiple sclerosis patients and normal individuals. In: Journal of Immunology. 1995 ; Vol. 154, No. 11. pp. 6191-6202.
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