TY - JOUR
T1 - Two distinct conformations of the conserved RNA-rich decoding center of the small ribosomal subunit are recognized by tRNAs and release factors
AU - Youngman, E. M.
AU - Cochella, L.
AU - Brunelle, J. L.
AU - He, S.
AU - Green, R.
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2006
Y1 - 2006
N2 - The mRNA is bound and poised in the decoding center of the small subunit of the ribosome where the genetic code is translated by the tRNAs, which recognize sense codons, and by the release factors, which recognize stop codons. Structural and biochemical studies have identified key universally conserved nucleotides, G530, A1492, and A1493, that are important for selection of cognate tRNA species during elongation. Here, we present evidence that these same universally conserved nucleotides are also important for interactions with the release factors, but must assume a very different structure during stopcodon recognition. These data provide mechanistic insight into how the decoding center of the ribosome has evolved to recognize distinct substrates with high fidelity, which in turn regulates the downstream chemical events of peptidyl transfer and peptide release.
AB - The mRNA is bound and poised in the decoding center of the small subunit of the ribosome where the genetic code is translated by the tRNAs, which recognize sense codons, and by the release factors, which recognize stop codons. Structural and biochemical studies have identified key universally conserved nucleotides, G530, A1492, and A1493, that are important for selection of cognate tRNA species during elongation. Here, we present evidence that these same universally conserved nucleotides are also important for interactions with the release factors, but must assume a very different structure during stopcodon recognition. These data provide mechanistic insight into how the decoding center of the ribosome has evolved to recognize distinct substrates with high fidelity, which in turn regulates the downstream chemical events of peptidyl transfer and peptide release.
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U2 - 10.1101/sqb.2006.71.036
DO - 10.1101/sqb.2006.71.036
M3 - Article
C2 - 17381338
AN - SCOPUS:34250648939
SN - 0091-7451
VL - 71
SP - 545
EP - 549
JO - Cold Spring Harbor symposia on quantitative biology
JF - Cold Spring Harbor symposia on quantitative biology
ER -