TY - JOUR
T1 - Two-dimensional iodopeptide mappoing demonstrates that erythrocyte Rh D, c, and E polypeptides are structurally homologous but nonidentical
AU - Blanchard, D.
AU - Bloy, C.
AU - Hermand, P.
AU - Cartron, J. P.
AU - Saboori, A. M.
AU - Smith, B. L.
AU - Agre, P.
PY - 1988
Y1 - 1988
N2 - The 32,000 molecular weight (mol wt) erythrocyte Rh, D, c, and E polypeptides were seperately purified from cDE/cDE erythrocytes by monoclonal immunoprecipitations and compared by two-dimensional iodopeptide mapping. Digestions of the isolated Rh polypeptides with α-chymotrypsin revealed a high degree of structural homology between c and E (13/14 iodopeptides were identical) and less striking homology between D and c or E (8/19 identical). The iodopeptide maps of Rh proteins purified by a nonimmunologic protocol from cDE/cDE erythrocytes were virtually identical to the composite pattern (D + c + E) deduced from the individual maps of Rh D, c, and E iodopeptides. Digestions of the isolated Rh polypeptides with trypsin revealed an overall homology of approximately 50% between iodopeptides derived from D, c, and E. These data indicate that the erythrocyte Rh D, c, and E antigens are carried by homologous but distinct molecular species; c and E appear more closely related to each other than to D.
AB - The 32,000 molecular weight (mol wt) erythrocyte Rh, D, c, and E polypeptides were seperately purified from cDE/cDE erythrocytes by monoclonal immunoprecipitations and compared by two-dimensional iodopeptide mapping. Digestions of the isolated Rh polypeptides with α-chymotrypsin revealed a high degree of structural homology between c and E (13/14 iodopeptides were identical) and less striking homology between D and c or E (8/19 identical). The iodopeptide maps of Rh proteins purified by a nonimmunologic protocol from cDE/cDE erythrocytes were virtually identical to the composite pattern (D + c + E) deduced from the individual maps of Rh D, c, and E iodopeptides. Digestions of the isolated Rh polypeptides with trypsin revealed an overall homology of approximately 50% between iodopeptides derived from D, c, and E. These data indicate that the erythrocyte Rh D, c, and E antigens are carried by homologous but distinct molecular species; c and E appear more closely related to each other than to D.
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U2 - 10.1182/blood.v72.4.1424.1424
DO - 10.1182/blood.v72.4.1424.1424
M3 - Article
C2 - 3139107
AN - SCOPUS:0023820510
SN - 0006-4971
VL - 72
SP - 1424
EP - 1427
JO - Blood
JF - Blood
IS - 4
ER -