Two-dimensional electrophoresis of human-CHO cell hybrids containing human chromosome 11

C. H. Scoggin, E. Gabrielson, J. N. Davidson, C. Jones, D. Patterson, T. T. Puck

Research output: Contribution to journalArticle

Abstract

Two-dimensional electrophoresis-electrofocusing with polyacrylamide gels is described in which the pattern of peptide spots obtained from hybrid cells of CHO containing the single human chromosome 11 are compared with those from the hybrid which has undergone reversion so as to lose this chromosome. At least eight distinct spots unique to the cell containing chromosome 11 have been located on the electrophoretogram. Experiments are described demonstrating applicability of the method to hybrids containing specific deletion mutants of chromosome 11, an approach which makes possible regional mapping of the loci responsible for specific peptides. The methodology appears applicable to study of gene expression under the influence of hormones and other agents and to the comparison of normal and disease situations.

Original languageEnglish (US)
Pages (from-to)389-398
Number of pages10
JournalSomatic Cell Genetics
Volume7
Issue number4
DOIs
StatePublished - Jul 1 1981
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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