Two antibodies that neutralize papillomavirus by different mechanisms show distinct binding patterns at 13 Å resolution

Frank P. Booy, Richard S Roden, Heather L. Greenstone, John T. Schiller, Benes L. Trus

Research output: Contribution to journalArticle

Abstract

Complexes between bovine papillomavirus type 1 (BPV1) and examples of two sets of neutralizing, monoclonal antibodies (mAb) to the major capsid protein (L1) were analyzed by low-dose cryo-electron microscopy and three-dimensional (3D) image reconstruction to 13 Å resolution. mAb 9 is representative of a set of neutralizing antibodies that can inhibit viral binding to the cell surface, while mAb 5B6 is representative of a second set that efficiently neutralizes papillomaviruses without significantly inhibiting viral binding to the cell surface. The 3D reconstructions reveal that mAb 9 binds to L1 molecules of both pentavalent and hexavalent capsomeres. In contrast, 5B6 binds only to hexavalent capsomeres, reflecting the significant structural or environmental differences for the 5B6 epitope in the 12 pentavalent capsomeres. Epitope localization shows that mAb 9 binds monovalently to the tips of capsomeres whereas 5B6 binds both monovalently and bivalently to the sides of hexavalent capsomeres approximately two-thirds of the way down from the outer tips, very close to the putative stabilizing intercapsomere connections. The absence of mAb 5B6 from the pentavalent capsomeres and its inability to prevent viral binding to the cell surface suggest that receptor binding may occur at one or more of the 12 virion vertices.

Original languageEnglish (US)
Pages (from-to)95-106
Number of pages12
JournalJournal of Molecular Biology
Volume281
Issue number1
DOIs
StatePublished - Aug 7 1998
Externally publishedYes

Fingerprint

Monoclonal Antibodies
Antibodies
Neutralizing Antibodies
Epitopes
Bovine papillomavirus 1
Cryoelectron Microscopy
Computer-Assisted Image Processing
Three-Dimensional Imaging
Capsid Proteins
Cell Surface Receptors
Virion

Keywords

  • 3D structure
  • Cryo-electron microscopy
  • Image reconstruction
  • Neutralizing antibodies
  • Papillomavirus

ASJC Scopus subject areas

  • Virology

Cite this

Two antibodies that neutralize papillomavirus by different mechanisms show distinct binding patterns at 13 Å resolution. / Booy, Frank P.; Roden, Richard S; Greenstone, Heather L.; Schiller, John T.; Trus, Benes L.

In: Journal of Molecular Biology, Vol. 281, No. 1, 07.08.1998, p. 95-106.

Research output: Contribution to journalArticle

Booy, Frank P. ; Roden, Richard S ; Greenstone, Heather L. ; Schiller, John T. ; Trus, Benes L. / Two antibodies that neutralize papillomavirus by different mechanisms show distinct binding patterns at 13 Å resolution. In: Journal of Molecular Biology. 1998 ; Vol. 281, No. 1. pp. 95-106.
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