Twist1 and Slug mediate H2AX-regulated epithelial-mesenchymal transition in breast cells

Urbain Weyemi, Christophe E. Redon, Taresh K. Sethi, Allison S. Burrell, Parthav Jailwala, Manjula Kasoji, Natalie Abrams, Anand Merchant, William M. Bonner

Research output: Contribution to journalComment/debatepeer-review

17 Scopus citations


The epithelial-mesenchymal transition (EMT) is thought to be essential for cancer metastasis. While chromatin remodeling is involved in EMT, which processes contribute to this remodeling remain poorly investigated. Recently, we showed that silencing or removal of the histone variant H2A.X induced mesenchymal-like characteristics, including activation of the EMT transcription factors, Slug and Zeb1 in human colon cancer cells. Here, we provide the evidence that H2A.X loss in human non-tumorigenic breast cell line MCF10A results in a robust EMT activation, as substantiated by a genome-wide expression analysis. Cells deficient for H2A.X exhibit enhanced migration and invasion, along with an activation of a set of mesenchymal genes and a concomitant repression of epithelial genes. In the breast model, the EMT-related transcription factor Twist1 cooperates with Slug to regulate EMT upon H2A.X Loss. Of interest, H2A.X expression level tightly correlates with Twist1, and to a lesser extent with Slug in the panel of human breast cancer cell lines of the NCI-60 datasets. These new findings indicate that H2A.X is involved in the EMT processes in cells of different origins but pairing with transcription factors for EMT may be tissue specific.

Original languageEnglish (US)
Pages (from-to)2398-2404
Number of pages7
JournalCell Cycle
Issue number18
StatePublished - Sep 16 2016


  • EMT
  • Slug
  • Twist1
  • breast cancer
  • histone H2AX

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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