Twist is a transcriptional repressor of E-cadherin gene expression in breast cancer

Farhad Vesuna, Paul van Diest, Ji Hshiung Chen, Venu Raman

Research output: Contribution to journalArticle

Abstract

Twist is a basic helix loop helix protein that plays a role both in human development and in cancer biogenesis. While characterizing the effects of Twist on breast epithelial cell transformation, we identified E-cadherin as a target gene that is down-regulated by Twist. In this study, we demonstrate that Twist can transcriptionally repress E-cadherin in breast cancer cells. Using transient promoter assays, we show that Twist can down-regulate E-cadherin promoter activity by up to two folds. This is further supported by immunoblot analyses which indicates that over-expression of Twist decreases E-cadherin protein levels in breast cancer cell lines. Subsequently, chromatin immunoprecipitation performed on MCF-7/Twist and Hs578 T (high level of endogenous Twist expression) confirmed Twist binding to the E-cadherin promoter. Finally, the functional relevance of this regulation was verified by quantitative real-time PCR and immunohistochemistry on a cohort of breast cancer samples.

Original languageEnglish (US)
Pages (from-to)235-241
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume367
Issue number2
DOIs
StatePublished - Mar 7 2008

Fingerprint

Cadherins
Gene expression
Breast Neoplasms
Gene Expression
Cells
Chromatin Immunoprecipitation
Human Development
Chromatin
Real-Time Polymerase Chain Reaction
Assays
Proteins
Breast
Down-Regulation
Genes
Epithelial Cells
Immunohistochemistry
Cell Line
Neoplasms

Keywords

  • Breast cancer
  • E-cadherin
  • Transcription regulation
  • Twist

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Twist is a transcriptional repressor of E-cadherin gene expression in breast cancer. / Vesuna, Farhad; van Diest, Paul; Chen, Ji Hshiung; Raman, Venu.

In: Biochemical and Biophysical Research Communications, Vol. 367, No. 2, 07.03.2008, p. 235-241.

Research output: Contribution to journalArticle

@article{c3744725f3a3424abac900a4eb0acc21,
title = "Twist is a transcriptional repressor of E-cadherin gene expression in breast cancer",
abstract = "Twist is a basic helix loop helix protein that plays a role both in human development and in cancer biogenesis. While characterizing the effects of Twist on breast epithelial cell transformation, we identified E-cadherin as a target gene that is down-regulated by Twist. In this study, we demonstrate that Twist can transcriptionally repress E-cadherin in breast cancer cells. Using transient promoter assays, we show that Twist can down-regulate E-cadherin promoter activity by up to two folds. This is further supported by immunoblot analyses which indicates that over-expression of Twist decreases E-cadherin protein levels in breast cancer cell lines. Subsequently, chromatin immunoprecipitation performed on MCF-7/Twist and Hs578 T (high level of endogenous Twist expression) confirmed Twist binding to the E-cadherin promoter. Finally, the functional relevance of this regulation was verified by quantitative real-time PCR and immunohistochemistry on a cohort of breast cancer samples.",
keywords = "Breast cancer, E-cadherin, Transcription regulation, Twist",
author = "Farhad Vesuna and {van Diest}, Paul and Chen, {Ji Hshiung} and Venu Raman",
year = "2008",
month = "3",
day = "7",
doi = "10.1016/j.bbrc.2007.11.151",
language = "English (US)",
volume = "367",
pages = "235--241",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Twist is a transcriptional repressor of E-cadherin gene expression in breast cancer

AU - Vesuna, Farhad

AU - van Diest, Paul

AU - Chen, Ji Hshiung

AU - Raman, Venu

PY - 2008/3/7

Y1 - 2008/3/7

N2 - Twist is a basic helix loop helix protein that plays a role both in human development and in cancer biogenesis. While characterizing the effects of Twist on breast epithelial cell transformation, we identified E-cadherin as a target gene that is down-regulated by Twist. In this study, we demonstrate that Twist can transcriptionally repress E-cadherin in breast cancer cells. Using transient promoter assays, we show that Twist can down-regulate E-cadherin promoter activity by up to two folds. This is further supported by immunoblot analyses which indicates that over-expression of Twist decreases E-cadherin protein levels in breast cancer cell lines. Subsequently, chromatin immunoprecipitation performed on MCF-7/Twist and Hs578 T (high level of endogenous Twist expression) confirmed Twist binding to the E-cadherin promoter. Finally, the functional relevance of this regulation was verified by quantitative real-time PCR and immunohistochemistry on a cohort of breast cancer samples.

AB - Twist is a basic helix loop helix protein that plays a role both in human development and in cancer biogenesis. While characterizing the effects of Twist on breast epithelial cell transformation, we identified E-cadherin as a target gene that is down-regulated by Twist. In this study, we demonstrate that Twist can transcriptionally repress E-cadherin in breast cancer cells. Using transient promoter assays, we show that Twist can down-regulate E-cadherin promoter activity by up to two folds. This is further supported by immunoblot analyses which indicates that over-expression of Twist decreases E-cadherin protein levels in breast cancer cell lines. Subsequently, chromatin immunoprecipitation performed on MCF-7/Twist and Hs578 T (high level of endogenous Twist expression) confirmed Twist binding to the E-cadherin promoter. Finally, the functional relevance of this regulation was verified by quantitative real-time PCR and immunohistochemistry on a cohort of breast cancer samples.

KW - Breast cancer

KW - E-cadherin

KW - Transcription regulation

KW - Twist

UR - http://www.scopus.com/inward/record.url?scp=38349023012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38349023012&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2007.11.151

DO - 10.1016/j.bbrc.2007.11.151

M3 - Article

VL - 367

SP - 235

EP - 241

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -