TY - JOUR
T1 - Turning of retinal growth cones in a netrin-1 gradient mediated by the netrin receptor DCC
AU - De La Torre, José R.
AU - Höpker, Veit H.
AU - Ming, Guo Li
AU - Poo, Mu Ming
AU - Tessier-Lavigne, Marc
AU - Hemmati-Brivanlou, Ali
AU - Holt, Christine E.
PY - 1997/12
Y1 - 1997/12
N2 - Netrin-1 promotes outgrowth of axons in vitro through the receptor Deleted in Colorectal Cancer (DCC) and elicits turning of axons within embryonic explants when presented as a point source. It is not known whether netrin-1 alone can elicit turning nor whether DCC mediates the turning response. We show that Xenopus retinal ganglion cell growth cones orient rapidly toward a pipette ejecting netrin-1, an effect blocked by antibodies to DCC. In vitro, netrin-1 induces a complex growth cone morphology reminiscent of that at the optic nerve head, a site of netrin-1 expression in vivo. These results demonstrate that netrin-1 can function alone to induce turning, implicate DCC in this response, and support the idea that netrin-1 contributes to steering axons out of the retina.
AB - Netrin-1 promotes outgrowth of axons in vitro through the receptor Deleted in Colorectal Cancer (DCC) and elicits turning of axons within embryonic explants when presented as a point source. It is not known whether netrin-1 alone can elicit turning nor whether DCC mediates the turning response. We show that Xenopus retinal ganglion cell growth cones orient rapidly toward a pipette ejecting netrin-1, an effect blocked by antibodies to DCC. In vitro, netrin-1 induces a complex growth cone morphology reminiscent of that at the optic nerve head, a site of netrin-1 expression in vivo. These results demonstrate that netrin-1 can function alone to induce turning, implicate DCC in this response, and support the idea that netrin-1 contributes to steering axons out of the retina.
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U2 - 10.1016/S0896-6273(00)80413-4
DO - 10.1016/S0896-6273(00)80413-4
M3 - Article
C2 - 9427245
AN - SCOPUS:0345404151
SN - 0896-6273
VL - 19
SP - 1211
EP - 1224
JO - Neuron
JF - Neuron
IS - 6
ER -