Tunneled superficial inferior epigastric artery (SIEA) myocutaneous/ vascularized femur chimeric flaps: A model to study the role of vascularized bone marrow in composite allografts

Gerhard S. Mundinger, Joseph A. Kelamis, Soon H. Kim, Michael Magarakis, Luke S. Jones, Jinny Ha, Eduardo D. Rodriguez

Research output: Contribution to journalArticle

Abstract

The role of vascularized bone marrow in promoting composite allograft survival can be assessed by intrinsically chimeric flaps. In this study, we introduce a significant modification to a previously described rat model of combined superficial inferior epigastric artery (SIEA) myocutaneous/vascularized femur transplantation. We previously noted autocannibalization in orthotopic myocutaneous SIEA allotransplants, which complicated clinical and histologic evaluation of rejection. We therefore designed syngeneic experiments in eight Lewis (RTl 1) rat pairs to explore the feasibility of tunneling the SIEA component of chimeric SIEA myocutaneous/vascularized femur flaps to the recipient dorsum. Vascularized SIEA myocutaneous/femur transplants survived in their entirety to POD 63 study endpoint with patent anastomoses in seven of eight (87.5%) transplants as confirmed clinically, histologically, and via near-infrared fluorescent angiography. Tunneling of the SIEA component of SIEA myocutaneous/vascularized femur flaps to the recipient dorsum can be achieved with high success rate and acceptable operative times, and is a technically easy method to study the role of vascularized bone marrow in composite allografts. This modification facilitates SIEA component monitoring, removes it from constant contact with cage bedding, and places it in a location where autocannibalization is unlikely.

Original languageEnglish (US)
Pages (from-to)128-135
Number of pages8
JournalMicrosurgery
Volume32
Issue number2
DOIs
StatePublished - Feb 2012

Fingerprint

Epigastric Arteries
Femur
Allografts
Bone Marrow
Transplants
Operative Time
Angiography
Transplantation

ASJC Scopus subject areas

  • Surgery

Cite this

Tunneled superficial inferior epigastric artery (SIEA) myocutaneous/ vascularized femur chimeric flaps : A model to study the role of vascularized bone marrow in composite allografts. / Mundinger, Gerhard S.; Kelamis, Joseph A.; Kim, Soon H.; Magarakis, Michael; Jones, Luke S.; Ha, Jinny; Rodriguez, Eduardo D.

In: Microsurgery, Vol. 32, No. 2, 02.2012, p. 128-135.

Research output: Contribution to journalArticle

Mundinger, Gerhard S. ; Kelamis, Joseph A. ; Kim, Soon H. ; Magarakis, Michael ; Jones, Luke S. ; Ha, Jinny ; Rodriguez, Eduardo D. / Tunneled superficial inferior epigastric artery (SIEA) myocutaneous/ vascularized femur chimeric flaps : A model to study the role of vascularized bone marrow in composite allografts. In: Microsurgery. 2012 ; Vol. 32, No. 2. pp. 128-135.
@article{4542087cc9b04b109b6a366a5926524b,
title = "Tunneled superficial inferior epigastric artery (SIEA) myocutaneous/ vascularized femur chimeric flaps: A model to study the role of vascularized bone marrow in composite allografts",
abstract = "The role of vascularized bone marrow in promoting composite allograft survival can be assessed by intrinsically chimeric flaps. In this study, we introduce a significant modification to a previously described rat model of combined superficial inferior epigastric artery (SIEA) myocutaneous/vascularized femur transplantation. We previously noted autocannibalization in orthotopic myocutaneous SIEA allotransplants, which complicated clinical and histologic evaluation of rejection. We therefore designed syngeneic experiments in eight Lewis (RTl 1) rat pairs to explore the feasibility of tunneling the SIEA component of chimeric SIEA myocutaneous/vascularized femur flaps to the recipient dorsum. Vascularized SIEA myocutaneous/femur transplants survived in their entirety to POD 63 study endpoint with patent anastomoses in seven of eight (87.5{\%}) transplants as confirmed clinically, histologically, and via near-infrared fluorescent angiography. Tunneling of the SIEA component of SIEA myocutaneous/vascularized femur flaps to the recipient dorsum can be achieved with high success rate and acceptable operative times, and is a technically easy method to study the role of vascularized bone marrow in composite allografts. This modification facilitates SIEA component monitoring, removes it from constant contact with cage bedding, and places it in a location where autocannibalization is unlikely.",
author = "Mundinger, {Gerhard S.} and Kelamis, {Joseph A.} and Kim, {Soon H.} and Michael Magarakis and Jones, {Luke S.} and Jinny Ha and Rodriguez, {Eduardo D.}",
year = "2012",
month = "2",
doi = "10.1002/micr.20957",
language = "English (US)",
volume = "32",
pages = "128--135",
journal = "Microsurgery",
issn = "0738-1085",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Tunneled superficial inferior epigastric artery (SIEA) myocutaneous/ vascularized femur chimeric flaps

T2 - A model to study the role of vascularized bone marrow in composite allografts

AU - Mundinger, Gerhard S.

AU - Kelamis, Joseph A.

AU - Kim, Soon H.

AU - Magarakis, Michael

AU - Jones, Luke S.

AU - Ha, Jinny

AU - Rodriguez, Eduardo D.

PY - 2012/2

Y1 - 2012/2

N2 - The role of vascularized bone marrow in promoting composite allograft survival can be assessed by intrinsically chimeric flaps. In this study, we introduce a significant modification to a previously described rat model of combined superficial inferior epigastric artery (SIEA) myocutaneous/vascularized femur transplantation. We previously noted autocannibalization in orthotopic myocutaneous SIEA allotransplants, which complicated clinical and histologic evaluation of rejection. We therefore designed syngeneic experiments in eight Lewis (RTl 1) rat pairs to explore the feasibility of tunneling the SIEA component of chimeric SIEA myocutaneous/vascularized femur flaps to the recipient dorsum. Vascularized SIEA myocutaneous/femur transplants survived in their entirety to POD 63 study endpoint with patent anastomoses in seven of eight (87.5%) transplants as confirmed clinically, histologically, and via near-infrared fluorescent angiography. Tunneling of the SIEA component of SIEA myocutaneous/vascularized femur flaps to the recipient dorsum can be achieved with high success rate and acceptable operative times, and is a technically easy method to study the role of vascularized bone marrow in composite allografts. This modification facilitates SIEA component monitoring, removes it from constant contact with cage bedding, and places it in a location where autocannibalization is unlikely.

AB - The role of vascularized bone marrow in promoting composite allograft survival can be assessed by intrinsically chimeric flaps. In this study, we introduce a significant modification to a previously described rat model of combined superficial inferior epigastric artery (SIEA) myocutaneous/vascularized femur transplantation. We previously noted autocannibalization in orthotopic myocutaneous SIEA allotransplants, which complicated clinical and histologic evaluation of rejection. We therefore designed syngeneic experiments in eight Lewis (RTl 1) rat pairs to explore the feasibility of tunneling the SIEA component of chimeric SIEA myocutaneous/vascularized femur flaps to the recipient dorsum. Vascularized SIEA myocutaneous/femur transplants survived in their entirety to POD 63 study endpoint with patent anastomoses in seven of eight (87.5%) transplants as confirmed clinically, histologically, and via near-infrared fluorescent angiography. Tunneling of the SIEA component of SIEA myocutaneous/vascularized femur flaps to the recipient dorsum can be achieved with high success rate and acceptable operative times, and is a technically easy method to study the role of vascularized bone marrow in composite allografts. This modification facilitates SIEA component monitoring, removes it from constant contact with cage bedding, and places it in a location where autocannibalization is unlikely.

UR - http://www.scopus.com/inward/record.url?scp=84856397961&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856397961&partnerID=8YFLogxK

U2 - 10.1002/micr.20957

DO - 10.1002/micr.20957

M3 - Article

C2 - 22113953

AN - SCOPUS:84856397961

VL - 32

SP - 128

EP - 135

JO - Microsurgery

JF - Microsurgery

SN - 0738-1085

IS - 2

ER -