Tumour necrosis factor-alpha and microalbuminuria in patients with inflammatory bowel disease

N. Mahmud, M. A. O'Connell, J. Stinson, M. G. Goggins, D. G. Weir, D. Kelleher

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To determine whether tumour necrosis factor-alpha (TNF-α) is important in the pathogenesis of microalbuminuria in patients with inflammatory bower disease (IBD). Patients and methods: We measured serum TNF-α, interleukin (IL)-6, the erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) levels and microalbuminuria in 48 patients with IBD. Serum TNF-α was measured by enzyme-linked immunosorbent assay and microalbuminuria was measured using an immunoturbiditimetric method. Clinical disease activity was quantified using the simple index of Harvey and Bradshaw. Results: Microalbuminuria was more severe in patients with IBD than in controls, and in patients with active versus inactive disease. TNF-α levels were higher in patients with IBD than in controls (mean ± SE 16.4 ± 1.4 versus 6.6 ± 1.3 pg/ml, respectively; P < 0.01) and in patients with active versus inactive IBD (means ± SE 20.1 ± 2 versus 12.8 ± 2.7 pg/ml; respectively P = 0.056). Microalbuminuria correlated strongly with TNF-α (r = 0.60; P < 0.009), ESR (r = 0.67, P < 0.02) and CRP levels (r = 0.935, P < 0.001). TNF-α correlated significantly with CRP (r = 0.54, P < 0.01). IL-6 levels were raised significantly in patients with IBD (7 ± 4 pg/ml, controls undetectable; P < 0.05). Patients with active IBD had higher IL-6 levels than those with inactive IBD (mean ± SE 13 ± 8 versus 0.90 ± 0.35 pg/ml, respectively; P < 0.05). However, IL-6 levels did not correlate with microalbuminuria in patients with IBD (r = 0.105, P = 0.256). Conclusion: Our findings suggest that TNF-α may be important in the pathogenesis of microalbuminuria in patients with IBD, possibly through TNF-induced damage to the glomerular basement membrane. The mechanism for this has not been defined but may relate to TNF-induced disruption of sulphated glycosaminoglycans.

Original languageEnglish (US)
Pages (from-to)215-219
Number of pages5
JournalEuropean Journal of Gastroenterology and Hepatology
Volume7
Issue number3
StatePublished - 1995
Externally publishedYes

Keywords

  • Crohn's disease
  • Inflammatory bowel disease
  • Interleukin-6
  • Microalbuminuria
  • Tumour necrosis factor
  • Ulcerative colitis

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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