Tumor suppressor gene P53 mutations in human prostate cancer

Yoshinobu Kubota, Taro Shuin, Hiroji Uemura, Kiyoshi Fujinami, Hiroshi Miyamoto, Soichiro Torigoe, Yasushi Dobashi, Hitoshi Kitamura, Yoshiko Iwasaki, Kathleen Danenberg, Peter V. Danenberg

Research output: Contribution to journalArticlepeer-review

Abstract

The genetic background underlying the growth and development of human prostatic cancer is not yet clear. Here we searched for possible mutations in the entire coding region of tumor suppressor gene p53 in primary human prostatic carcinomas, using polymerase chain reaction and single‐strand conformational polymorphism analysis of RNA. We found p53 gene mutations in 4 of 21 cases (19%). DNA sequencing of the polymerase chain reaction products revealed missense point mutations that resulted in amino acid changes in exon 5 or 3 in three cases and single base deletions in exon 7 in two cases. One case contained both a missense point mutation and a single base deletion. Three of these four cases were pathologically diagnosed as poorly differentiated adenocarcinomas, and three of the four cases were clinically localized to stage C or D. None of seven noncancerous prostate tissues nor three well‐differentiated adenocarcinoma tissues showed any mutations. The present results suggest that p53 gene mutation is involved in the late progression steps of human prostate carcinogenesis. © 1995 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)18-24
Number of pages7
JournalThe Prostate
Volume27
Issue number1
DOIs
StatePublished - Jul 1995

Keywords

  • PCR
  • RNA‐SSCP analysis
  • human prostate cancer
  • p53 gene

ASJC Scopus subject areas

  • Oncology
  • Urology

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