Tumor suppressor BLU enhances pro-apoptotic activity of sMEK1 through physical interaction

Seung Myung Dong, Hyun Jung Byun, Boh Ram Kim, Seung Hoon Lee, Barry Trink, Seung Bae Rho

Research output: Contribution to journalArticlepeer-review

Abstract

BLU is a tumor suppressor that acts as a transcriptional regulator through the association with cellular components. However, the working mechanism of BLU in cellular functions was not understood. We found that BLU directly interacts with sMEK1, a regulatory subunit of protein phosphatase 4. Furthermore, we determined the binding domains that are required for interaction between BLU and sMEK1. The N-terminal of BLU was observed to interact with the C-terminal of sMEK1. Binding activity was confirmed by the BLU-dependent increase of sMEK1 expression, as well as by the induced apoptotic activity. Also, expression of BLU and sMEK1 was down-regulated in ovarian and cervical patients, and was hypermethylated. These findings indicate that BLU can mediate the pro-apoptotic activity through the induction of sMEK1.

Original languageEnglish (US)
Pages (from-to)1208-1214
Number of pages7
JournalCellular Signalling
Volume24
Issue number6
DOIs
StatePublished - Jun 1 2012

Keywords

  • BLU
  • Cell cycle
  • Cervical tumorigenesis
  • Pro-apoptosis
  • Protein-protein interaction
  • SMEK1

ASJC Scopus subject areas

  • Cell Biology

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