Tumor suppressor BLU enhances pro-apoptotic activity of sMEK1 through physical interaction

Seung Myung Dong, Hyun Jung Byun, Boh Ram Kim, Seung Hoon Lee, Barry Trink, Seung Bae Rho

Research output: Contribution to journalArticle

Abstract

BLU is a tumor suppressor that acts as a transcriptional regulator through the association with cellular components. However, the working mechanism of BLU in cellular functions was not understood. We found that BLU directly interacts with sMEK1, a regulatory subunit of protein phosphatase 4. Furthermore, we determined the binding domains that are required for interaction between BLU and sMEK1. The N-terminal of BLU was observed to interact with the C-terminal of sMEK1. Binding activity was confirmed by the BLU-dependent increase of sMEK1 expression, as well as by the induced apoptotic activity. Also, expression of BLU and sMEK1 was down-regulated in ovarian and cervical patients, and was hypermethylated. These findings indicate that BLU can mediate the pro-apoptotic activity through the induction of sMEK1.

Original languageEnglish (US)
Pages (from-to)1208-1214
Number of pages7
JournalCellular Signalling
Volume24
Issue number6
DOIs
StatePublished - Jun 2012

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Neoplasms
protein phosphatase 4

Keywords

  • BLU
  • Cell cycle
  • Cervical tumorigenesis
  • Pro-apoptosis
  • Protein-protein interaction
  • SMEK1

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dong, S. M., Byun, H. J., Kim, B. R., Lee, S. H., Trink, B., & Rho, S. B. (2012). Tumor suppressor BLU enhances pro-apoptotic activity of sMEK1 through physical interaction. Cellular Signalling, 24(6), 1208-1214. https://doi.org/10.1016/j.cellsig.2012.02.002

Tumor suppressor BLU enhances pro-apoptotic activity of sMEK1 through physical interaction. / Dong, Seung Myung; Byun, Hyun Jung; Kim, Boh Ram; Lee, Seung Hoon; Trink, Barry; Rho, Seung Bae.

In: Cellular Signalling, Vol. 24, No. 6, 06.2012, p. 1208-1214.

Research output: Contribution to journalArticle

Dong, Seung Myung ; Byun, Hyun Jung ; Kim, Boh Ram ; Lee, Seung Hoon ; Trink, Barry ; Rho, Seung Bae. / Tumor suppressor BLU enhances pro-apoptotic activity of sMEK1 through physical interaction. In: Cellular Signalling. 2012 ; Vol. 24, No. 6. pp. 1208-1214.
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