@article{e76ab0574ea0496da2c0c30d54670c6f,
title = "Tumor-specific imaging through progression elevated gene-3 promoter-driven gene expression",
abstract = "Molecular-genetic imaging is advancing from a valuable preclinical tool to a guide for patient management. The strategy involves pairing an imaging reporter gene with a complementary imaging agent in a system that can be used to measure gene expression or protein interaction or track gene-tagged cells in vivo. Tissue-specific promoters can be used to delineate gene expression in certain tissues, particularly when coupled with an appropriate amplification mechanism. Here we show that the progression elevated gene-3 (PEG-3) promoter, derived from a rodent gene mediating tumor progression and metastatic phenotypes, can be used to drive imaging reporters selectively to enable detection of micrometastatic disease in mouse models of human melanoma and breast cancer using bioluminescence and radionuclide-based molecular imaging techniques. Because of its strong promoter activity, tumor specificity and capacity for clinical translation, PEG-3 promoter-driven gene expression may represent a practical, new system for facilitating cancer imaging and therapy.",
author = "Bhang, {Hyo Eun C.} and Gabrielson, {Kathleen L.} and John Laterra and Fisher, {Paul B.} and Pomper, {Martin G.}",
note = "Funding Information: We appreciate the technical support provided by S. Dhara, S. Nimmagadda, M. Goggins and M. Griffith. We are grateful to J. Fox and G. Green for providing [125I]FIAU and assisting in the SPECT-CT and PET-CT imaging studies. We also thank C. Endres, B. Tsui, J. Yu and J. Fox for help with SPECT and PET data processing. The MDA-MB-231 cell line and pCMV-Tri construct were generous gifts from Z. Bhujwalla (Johns Hopkins University) and S. Gambhir (Stanford University), respectively. Funding was provided by US National Institutes of Health grant U24 CA92871 (to M.G.P.), by the Predoctoral Molecular Imaging Funding Information: Scholar Program from the Society of Nuclear Medicine and the Korea Science and Engineering Foundation Fellowship Program (to H.C.B.) and by the US National Institutes of Health grant P01 CA104177 and the US National Foundation for Cancer Research (to P.B.F.). P.B.F. holds the Thelma Newmeyer Corman Chair in Cancer Research at the Virginia Commonwealth University Massey Cancer Center.",
year = "2011",
month = jan,
doi = "10.1038/nm.2269",
language = "English (US)",
volume = "17",
pages = "123--129",
journal = "Nature medicine",
issn = "1078-8956",
publisher = "Nature Publishing Group",
number = "1",
}