Tumor Protein (TP)-p53 members as regulators of autophagy in tumor cells upon marine drug exposure

Edward A. Ratovitski, Friedemann Honecker, Sergey A. Dyshlovoy

Research output: Contribution to journalArticlepeer-review

Abstract

Targeting autophagic pathways might play a critical role in designing novel chemotherapeutic approaches in the treatment of human cancers, and the prevention of tumor-derived chemoresistance. Marine compounds were found to decrease tumor cell growth in vitro and in vivo. Some of them were shown to induce autophagic flux in tumor cells. In this study, we observed that the selected marine life-derived compounds (Chromomycin A2, Psammaplin A, and Ilimaquinone) induce expression of several autophagic signaling intermediates in human squamous cell carcinoma, glioblastoma, and colorectal carcinoma cells in vitro through a transcriptional regulation by tumor protein (TP)-p53 family members. These conclusions were supported by specific qPCR expression analysis, luciferase reporter promoter assay, and chromatin immunoprecipitation of promoter sequences bound to the TP53 family proteins, and silencing of the TP53 members in tumor cells.

Original languageEnglish (US)
Article number154
JournalMarine Drugs
Volume14
Issue number8
DOIs
StatePublished - Aug 1 2016

Keywords

  • Autophagy
  • Cancer
  • Marine drugs
  • P53 family members
  • Transcription

ASJC Scopus subject areas

  • Drug Discovery

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