Tumor necrosis factor α promotes nuclear localization of cytokine- inducible CCAAT/enhancer binding protein isoforms in hepatocytes

Ming Yin, Shi Qui Yang, Hui Zhi Lin, M. Daniel Lane, Subroto B Chatterjee, Anna Mae Diehl

Research output: Contribution to journalArticle

Abstract

Hepatocytes were cultured in the presence of recombinant tumor necrosis factor (TNF) α or mutated TNF α peptides that specifically activate either p55 or p75 TNF receptors to determine if TNF α can activate cytokine- inducible CCAAT/enhancer binding protein (C/EBP) isoforms by post- transcriptional mechanisms that are initiated by TNF receptors. Within 5-10 min after treatment with any of these agents, nuclear concentrations of C/EBP β and C/EBP δ double and remain 2-4-fold greater than control cultures for 30 min (p <0.01). Consistent with these results, gel mobility shift assays demonstrate 3-fold increased nuclear C/EBP β- and C/EBP δ-DNA binding activity in TNF α-treated cells, and immunocytochemistry confirms rapid redistribution of these C/EBP isoforms into the nucleus. In contrast, mRNA and whole cell protein concentrations of C/EBP β and δ are not altered by TNF α exposure, and nuclear concentrations of another C/EBP isoform, C/EBP α, are decreased by 80%. This novel evidence that TNF α initiates post- transcriptional activation of cytokine-inducible C/EBP isoforms identifies a mechanism that enables hepatocytes to respond immediately to inflammatory stress.

Original languageEnglish (US)
Pages (from-to)17974-17978
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number30
DOIs
StatePublished - 1996

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CCAAT-Enhancer-Binding Proteins
Hepatocytes
Protein Isoforms
Tumor Necrosis Factor-alpha
Cytokines
Tumor Necrosis Factor Receptors
Electrophoretic Mobility Shift Assay
Nuclear Proteins
Protein Binding
Transcriptional Activation
Assays
Gels
Chemical activation
Immunohistochemistry
Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Tumor necrosis factor α promotes nuclear localization of cytokine- inducible CCAAT/enhancer binding protein isoforms in hepatocytes. / Yin, Ming; Yang, Shi Qui; Lin, Hui Zhi; Daniel Lane, M.; Chatterjee, Subroto B; Diehl, Anna Mae.

In: Journal of Biological Chemistry, Vol. 271, No. 30, 1996, p. 17974-17978.

Research output: Contribution to journalArticle

Yin, Ming ; Yang, Shi Qui ; Lin, Hui Zhi ; Daniel Lane, M. ; Chatterjee, Subroto B ; Diehl, Anna Mae. / Tumor necrosis factor α promotes nuclear localization of cytokine- inducible CCAAT/enhancer binding protein isoforms in hepatocytes. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 30. pp. 17974-17978.
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AU - Diehl, Anna Mae

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