Tumor necrosis factor α induces expression of human immunodeficiency virus in a chronically infected T-cell clone

T. M. Folks, K. A. Clouse, J. Justement, A. Rabson, Elia J Duh, J. H. Kehrl, A. S. Fauci

Research output: Contribution to journalArticle

Abstract

Tumor necrosis factor α (TNF-α), also known as cachectin, was demonstrated to induce the expression of human immunodeficiency virus (HIV) in a chronically infected T-cell clone (ACH-2). Concentrations of recombinant TNF-α as low as 50 pg/ml induced a significant increase over background of HIV expression in the ACH-2 cells as determined by supernatant reverse transcriptase activity. The HIV-inducing effects of TNF-α could not be explained by toxic effects on the cells. In addition, both the uninfected parental cell line (A3.01) and the infected ACH-2 cells were shown to have high-affinity receptors for TNF-α. Transient-transfection experiments demonstrated that the inductive effects of TNF-α were due to specific activation of the HIV long terminal repeat. These studies provide evidence that TNF-α may play a role in the mechanisms of pathogenesis of HIV infection.

Original languageEnglish (US)
Pages (from-to)2365-2368
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number7
StatePublished - 1989
Externally publishedYes

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Clone Cells
Tumor Necrosis Factor-alpha
HIV
T-Lymphocytes
HIV Long Terminal Repeat
Tumor Necrosis Factor Receptors
Poisons
RNA-Directed DNA Polymerase
Virus Diseases
Transfection
Cell Line

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Tumor necrosis factor α induces expression of human immunodeficiency virus in a chronically infected T-cell clone. / Folks, T. M.; Clouse, K. A.; Justement, J.; Rabson, A.; Duh, Elia J; Kehrl, J. H.; Fauci, A. S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 7, 1989, p. 2365-2368.

Research output: Contribution to journalArticle

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AU - Folks, T. M.

AU - Clouse, K. A.

AU - Justement, J.

AU - Rabson, A.

AU - Duh, Elia J

AU - Kehrl, J. H.

AU - Fauci, A. S.

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