Tumor necrosis factor α and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor κB

L. Osborn, S. Kunkel, G. J. Nabel

Research output: Contribution to journalArticle

Abstract

Binding of peptide hormones to surface membrane receptors leads to the transcription of specific genes within relevant target cells. How these signals are transduced to alter gene expression is largely unknown, but this mechanism probably involves a sequence of enzymatic steps that activate factors in the nucleus that modulate transcription. We now demonstrate that two different peptide hormones, or cytokines, stimulate the human immunodeficiency virus enhancer, and this effect is mediated by nuclear factor (NF) κB (nuclear factor that binds the κ immunoglobulin light chain gene enhancer). These cytokines, tumor necrosis factor α and interleukin 1, act on multiple cell types and represent the only naturally occurring activators of this transcription factor among eight cytokines examined. Although NF-κB binding can be stimulated by phorbol 12-myristate 13-acetate, tumor necrosis factor α acts through an independent mechanism, inducing NF-κB binding in HT-2-cells, which did not show increased binding in response to phorbol 12-myristate 13-acetate, and causing superinduction in Jurkat T-lymphoma cells. Tumor necrosis factor α is also a more selective activator of T cells than phorbol 12-myristate 13-acetate, having no effect on lymphokine production in EL-4 cells at the same time it induces NF-κB. These findings suggest that human immunodeficiency virus gene expression can be induced in T cells without activating lymphokine secretion and that the role of these cytokines in the activation of latent human immunodeficiency virus infection deserves further clinical evaluation. Finally, this link between binding at the surface membrane and stimulation of a specific transcription factor should help define intermediates for these cytokine activation pathways.

Original languageEnglish (US)
Pages (from-to)2336-2340
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number7
StatePublished - 1989
Externally publishedYes

Fingerprint

Virus Activation
Interleukin-1
Tumor Necrosis Factor-alpha
HIV
Cytokines
Acetates
Peptide Hormones
Lymphokines
Immunoglobulin Light Chain Genes
Transcription Factors
T-Lymphocytes
Gene Expression
Membranes
T-Cell Lymphoma
Virus Diseases
Genes
phorbol-12-myristate

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

@article{a8efc11daff24adc86adf50adf12af45,
title = "Tumor necrosis factor α and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor κB",
abstract = "Binding of peptide hormones to surface membrane receptors leads to the transcription of specific genes within relevant target cells. How these signals are transduced to alter gene expression is largely unknown, but this mechanism probably involves a sequence of enzymatic steps that activate factors in the nucleus that modulate transcription. We now demonstrate that two different peptide hormones, or cytokines, stimulate the human immunodeficiency virus enhancer, and this effect is mediated by nuclear factor (NF) κB (nuclear factor that binds the κ immunoglobulin light chain gene enhancer). These cytokines, tumor necrosis factor α and interleukin 1, act on multiple cell types and represent the only naturally occurring activators of this transcription factor among eight cytokines examined. Although NF-κB binding can be stimulated by phorbol 12-myristate 13-acetate, tumor necrosis factor α acts through an independent mechanism, inducing NF-κB binding in HT-2-cells, which did not show increased binding in response to phorbol 12-myristate 13-acetate, and causing superinduction in Jurkat T-lymphoma cells. Tumor necrosis factor α is also a more selective activator of T cells than phorbol 12-myristate 13-acetate, having no effect on lymphokine production in EL-4 cells at the same time it induces NF-κB. These findings suggest that human immunodeficiency virus gene expression can be induced in T cells without activating lymphokine secretion and that the role of these cytokines in the activation of latent human immunodeficiency virus infection deserves further clinical evaluation. Finally, this link between binding at the surface membrane and stimulation of a specific transcription factor should help define intermediates for these cytokine activation pathways.",
author = "L. Osborn and S. Kunkel and Nabel, {G. J.}",
year = "1989",
language = "English (US)",
volume = "86",
pages = "2336--2340",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "7",

}

TY - JOUR

T1 - Tumor necrosis factor α and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor κB

AU - Osborn, L.

AU - Kunkel, S.

AU - Nabel, G. J.

PY - 1989

Y1 - 1989

N2 - Binding of peptide hormones to surface membrane receptors leads to the transcription of specific genes within relevant target cells. How these signals are transduced to alter gene expression is largely unknown, but this mechanism probably involves a sequence of enzymatic steps that activate factors in the nucleus that modulate transcription. We now demonstrate that two different peptide hormones, or cytokines, stimulate the human immunodeficiency virus enhancer, and this effect is mediated by nuclear factor (NF) κB (nuclear factor that binds the κ immunoglobulin light chain gene enhancer). These cytokines, tumor necrosis factor α and interleukin 1, act on multiple cell types and represent the only naturally occurring activators of this transcription factor among eight cytokines examined. Although NF-κB binding can be stimulated by phorbol 12-myristate 13-acetate, tumor necrosis factor α acts through an independent mechanism, inducing NF-κB binding in HT-2-cells, which did not show increased binding in response to phorbol 12-myristate 13-acetate, and causing superinduction in Jurkat T-lymphoma cells. Tumor necrosis factor α is also a more selective activator of T cells than phorbol 12-myristate 13-acetate, having no effect on lymphokine production in EL-4 cells at the same time it induces NF-κB. These findings suggest that human immunodeficiency virus gene expression can be induced in T cells without activating lymphokine secretion and that the role of these cytokines in the activation of latent human immunodeficiency virus infection deserves further clinical evaluation. Finally, this link between binding at the surface membrane and stimulation of a specific transcription factor should help define intermediates for these cytokine activation pathways.

AB - Binding of peptide hormones to surface membrane receptors leads to the transcription of specific genes within relevant target cells. How these signals are transduced to alter gene expression is largely unknown, but this mechanism probably involves a sequence of enzymatic steps that activate factors in the nucleus that modulate transcription. We now demonstrate that two different peptide hormones, or cytokines, stimulate the human immunodeficiency virus enhancer, and this effect is mediated by nuclear factor (NF) κB (nuclear factor that binds the κ immunoglobulin light chain gene enhancer). These cytokines, tumor necrosis factor α and interleukin 1, act on multiple cell types and represent the only naturally occurring activators of this transcription factor among eight cytokines examined. Although NF-κB binding can be stimulated by phorbol 12-myristate 13-acetate, tumor necrosis factor α acts through an independent mechanism, inducing NF-κB binding in HT-2-cells, which did not show increased binding in response to phorbol 12-myristate 13-acetate, and causing superinduction in Jurkat T-lymphoma cells. Tumor necrosis factor α is also a more selective activator of T cells than phorbol 12-myristate 13-acetate, having no effect on lymphokine production in EL-4 cells at the same time it induces NF-κB. These findings suggest that human immunodeficiency virus gene expression can be induced in T cells without activating lymphokine secretion and that the role of these cytokines in the activation of latent human immunodeficiency virus infection deserves further clinical evaluation. Finally, this link between binding at the surface membrane and stimulation of a specific transcription factor should help define intermediates for these cytokine activation pathways.

UR - http://www.scopus.com/inward/record.url?scp=0342742310&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0342742310&partnerID=8YFLogxK

M3 - Article

C2 - 2494664

AN - SCOPUS:0342742310

VL - 86

SP - 2336

EP - 2340

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 7

ER -