Abstract
Expression of human immunodeficiency virus type 1 (HIV-1) can be activated in a chronically infected T-cell line (ACH2 cells) by a cytokine, human tumor necrosis factor α (TNF-α). TNF-α treatment of ACH2 cells resulted in an increase in steady-state levels of HIV RNA and HIV transcription. Gel mobility shift assays demonstrated that the transcriptional activation of the HIV long terminal repeat (LTR) by TNF-α was associated with the induction of a nuclear factor(s) binding to the NF-κB sites in the LTR. Deletion of the NF-κB sites from the LTR eliminated activation by TNF-α in T cells transfected with plasmids in which the HIV LTR directed the expression of the bacterial chloramphenicol acetyltransferase gene. Thus, TNF-α appears to activate HIV RNA and virus production by aCH2 cells through the induction of transcription-activating factors that bind to the NF-κB sequences in the HIV LTR.
Original language | English (US) |
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Pages (from-to) | 5974-5978 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 86 |
Issue number | 15 |
DOIs | |
State | Published - 1989 |
Externally published | Yes |
ASJC Scopus subject areas
- General